Non-covalent ligands

Schwartz, B. L., Gale, D. C., Smith, R. D., Chilkoti, A., and Stayton, P. S. (1995). Investigation of non-covalent ligand binding to the intact strepavidin tetramer by electrospray ionization mass spectrometry. J. Mass Spectrom. 30, 1095-1102. 

Huang H, Melacini G. High-resolution protein hydration NMR experiments probing how protein surfaces interact with water and other non-covalent ligands. Analyt. Chim. Acta 2006 564 1-9. 

The identification of ligands for targeting the AR and the ER was simplified due to the existence of natural high-affinity non-covalent ligands for both... 

Generally, non-denaturing conditions are anployed when protein subunit stoichiometry or non-covalent ligand binding are being measured, as the native protein fold is often required for such functionality. Whereas denaturing conditions offer increased signal intensity and reduced peak adduction, ideal for an accurate mass measurement. 

Non-covalently Immobilized Catalysts Based on Chiral Salen Ligands. . 152... 

This spectroscopy has been used as a sensitive and effective analytical technique for the characterization of specific ligand-oligonucleotide duplex non-covalent complexes [138], due to its specificity, sensitivity and quickness as well as its advantage in determining binding stoichiometry. 

An alternative way for the generation of a bidentate ligand makes use of a self-assembly process of monodentate to bidentate ligands employing non-covalent interactions [87]. 

Two-dimensional planar interpenetrating networks have been formed using the spacer ligand 2,2 -bis-l,6-naphthyridine with a zinc salt.274 Helicate structures have been synthesized which rely heavily on non-covalent interactions in the metal-assisted self-assembly process in solution.275... 

G. Thoma, M. B. Streiff, A. G. Katopodis, R. O. Duthaler, N. H. Voelcker, C. Ehrhardt, and C. Masson, Non-covalent polyvalent ligands by self-assembly of small glycodendrimers A novel concept for the inhibition of polyvalent carbohydrate-protein interactions in vitro and in vivo, Chem. Eur. J., 12 (2006) 99-117. 

A non-covalently functionalized dendrimer was also applied in a continuous allylic amination reaction.[33] PPI dendrimers functionalized with urea adamantyl groups can act as host molecules for phosphorus ligands equipped with acetyl urea groups (Figure 4.18). The so formed supramolecular complex was reacted with a palladium precursor... 

In the classical photoaffinity experiment a tissue homogenate is solubilized by standard detergents and incubated with the radiolabeled photoprobe for non-covalent binding prior to irradiation. By illumination at the excitation wavelength a covalent linkage is established between the ligand and receptor around the binding site as shown in Scheme 2. 

The mechanism shown in Scheme 5 postulates the formation of a Fe(II)-semi-quinone intermediate. The attack of 02 on the substrate generates a peroxy radical which is reduced by the Fe(II) center to produce the Fe(III) peroxide complex. The semi-quinone character of the [FeL(DTBC)] complexes is clearly determined by the covalency of the iron(III)-catechol bond which is enhanced by increasing the Lewis acidity of the metal center. Thus, ultimately the non-participating ligand controls the extent of the Fe(II) - semi-quinone formation and the rate of the reaction provided that the rate-determining step is the reaction of 02 with the semiquinone intermediate. In the final stage, the substrate is oxygenated simultaneously with the release of the FemL complex. An alternative model, in which 02 attacks the Fe(II) center instead of the semi-quinone, cannot be excluded either. 

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