Non-chromatographic

Henderson SR, Baker C, Fink G (1977) Oestradiol-17beta and pituitary responsiveness to luteinizing hormone releasing factor in the rat a study using rectangular pulses of oestradiol-17 beta monitored by non-chromatographic radioimmunoassay. J Endocrinol 73 441-453... 

Commercial mixtures of surfactants comprise several tens to hundreds of homologues, oligomers and isomers of anionic, nonionic, cationic and amphoteric compounds. Therefore, their identification and quantification in the environment is complicated and cumbersome. The requirement of more specific analytical methods has prompted a replacement of many of the separate steps in traditional methods of analysis, usually non-chromatographic, by chromatographic tools. 

In addition to online resources, other texts and references have discussed the process of validation for methods used in the pharmaceutical industry in relation to the regulatory guidance documents. These guides include discussions on method development in relation to method validation, the validation of non-chromatographic methods and stability indicating methods. 

Valcarcel, M. Luque de Castro, M. Non chromatographic Continuous Separation Techniques, Royal Society of Chemistry Cambridge, UK, 1990. Karlberg, B. Thelander, S. Anal. Chim. Acta., 1978, 98, 1. 

M. VALCARCEL and M.D. LUQUE DE CASTRO, "Non-Chromatographic Continuous Separation Techniques", Royal Society of Chemistry, Cambridge, 1991. 

For a detailed description of the separation processes that may take place at the sensing microzone, the foundation of which is closely related to non-chromatographic continuous separation techniques based on mass transfer across a gas-liquid (gas diffusion), liquid-liquid (dialysis, ultrafiltration) or liquid-solid interface (sorption), interested readers are referred to specialized monographs e.g. [3]). 

Is it possible to start with non-chromatographic procedures, e.g., centrifugation ... 

Miscellaneous Non-Chromatographic Methods of Analysis Chromatographic Methods of Analysis... 

The interferences appeared to be similar to those of Bean et al. (10). This observation suggested that high molecular weight, non-chromatographable materials were present in the ether concentrate from the XAD resin accumulator. The dramatic effects of the interferences were not anticipated because previous XAD-2 resin accumulator analyses by packed-column GC reported by Suffet et al. (2) and Yohe et al (5) showed acceptable results. 

Humic Substance Interference. The probable interferences within the sample matrix were thought to be high molecular weight, non-chromatographable materials called humic substances. This conclusion was reached on the basis of (1) the results of the Grob column-performance text mix, (2) the type of successful on-column chemical cleanup, and (3) a review of the literature regarding the nonchromatographable constituents of natural waters. 

The sample cleanup procedure is efficient and easy to perform. The cleanup procedure removes approximately 84% of the non-chromatographable materials. The losses from the sample affect only a small number of the synthetic organic chemicals of interest in the field of drinking water treatment. The compounds undergoing the largest losses were phthalates and diacetone sugars. 

Contents 1. Introduction 160 2. Methods to Achieve High-Resolution NMR Spectra in the Presence of Delaying Media 161 2.1. MAS for magnetic susceptibility averaging 161 2.2. Chrom-NMR without MAS 162 3. Mobility PFG 162 3.1. Diffusion NMR and its representation 162 3.2. Diffusion in multiphasic environments 164 3.3. Specificity of diffusion PFG measurements in porous media 165 4. Examples of Chrom-NMR 166 4.1. Comparison with Liquid Chromatography (LC) 166 4.2. Non-chromatographic mobile phases 169 5. Conclusions 171 Acknowledgements 172 References 172... 

Another example of the use of non-chromatographic solvents was mentioned above, as a mixture susceptibility matched to silica, and it is... 

Szente L, Szejtli J. Non-chromatographic analytical uses of cyclodextrins. Analyst 1998 123 735-741. 

Describe a non-chromatographic method for the separation of the enantiomers of rac- 1-phenylethanamine. 

McKinney, M. M., and Parkinson, A. (1987). A simple, non-chromatographic procedure to purify immunoglobulins from serum and ascites fluid. J. Immunol. Methods 96, 271-278. 

The central cavity of the cylinder-shaped cyclodextrins behaves as an empty capsule It can accommodate so-called guest molecules of appropriate size, shape, and polarity. This "molecular encapsulation" can be utilized for stabilization and for enhancement of solubility of drugs, vitamins, flavors, etc., and utilizing the selectivity of the inclusion complexation, it can be applied for separation of substances, either by non-chromatographic methods, or chromatographic methods. 

A characteristic feature of non-chromatographic separations utilizing cyclodextrins is that they are aimed at preparative separations. Unfortunately only incomplete separations or enrichments can be attained. By repeating the separations in multistage processes, the required component can be enriched on preparative, and even industrial, scale. Many examples have been published both for partial separation of compounds, isomers, or enantiomers through selective crystallization of their complexes (3). 

A large number of studies on non-chromatographic methods of surface separation of dissolved substances (molecules and ions) and highly dispersed particles (colloids and other small particles) to which the principles of common flotation are inapplicable, have been... 

A simpler desaiption of the system is desired and it can be obtained using equilibrium constants that describe aU eqnilibria in the systan obviously only a thermodynamic equilibrium constant must be made use of since, as discussed above, at variance with stoichiometric constants, they account for all the molecular level interactions in a very simple way, and notably, their chromatographic and non-chromatographic estimates can be compared to validate the retention mechanism they describe. 

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