NMR approaches

Kitamaru, R. and Uorii, F. NMR Approach to the Phase Structure of Linear Polyethylene. 

Eckert H,Elbers S,Epping JD, Janssen M, Kalwei M, StrojekW, Voigt U (2005) Dipolar Solid State NMR Approaches Towards Medium-Range Structure in Oxide Glasses. 246 195-233... 

Fig. 3 The SAR by NMR approach. Example of a small bidentate molecule designed using this approach. The example shown is for the design of a potent inhibitors of the matrix metalloproteinase MMP3. (a) Docked structures of the identified fragment leads are shown with cyan carbons, whereas the linked compound is shown with green carbon atoms. All structures were experimentally determined by NMR. (b) Chemical structures (and in vitro potencies) of the fragment leads and subsequent high-affinity linked compounds. Adapted from [7]...

J.P. Cohen-Addad, NMR approach to siloxane properties Melts, gels, silica mixtures. In S.J. Clarson and J.A. Semiyen (Eds.), Siloxane Polymers, Prentice Hall, Englewood Cliffs, 1993, p. 511. 

A well-known and established NMR approach is CP-MAS, which provides good sensitivity and spectral resolution for both weak and strong interacting ligands. In fact, it is possible to distinguish the free ligand and the bound to the membrane on the basis of their differing dynamics. The polarization transfer from the 1H to 13C is mediated by the... 

Here, we discuss a solid-state 19F-NMR approach that has been developed for structural studies of MAPs in lipid bilayers, and how this can be translated to measurements in native biomembranes. We review the essentials of the methodology and discuss key objectives in the practice of 19F-labelling of peptides. Furthermore, the preparation of macroscopically oriented biomembranes on solid supports is discussed in the context of other membrane models. Two native biomembrane systems are presented as examples human erythrocyte ghosts as representatives of eukaryotic cell membranes, and protoplasts from Micrococcus luteus as membranes... 

Answer. There has been little effective interplay between experimental results obtained on single nanostructures grown as quantum-wells and studied by optical-pumping methods and those obtained on bulk nanoscale semiconductors by more conventional NMR approaches. However, this situation may change, since the former studies can provide information about the effects of, e.g., charge carriers or strain or compositional interfaces upon NMR parameters such as chemical and Knight shifts and EFGs in reasonably well-defined systems. 

A complete NMR approach has been employed to evaluate the complexation process of catechin A with p-CD and synthetic analogues.125 The analysis of the variation of the proton chemical shifts indicated the formation of a 1 1 stoichiometric complex. 2D-ROESY provided detailed spatial information of the complex while the binding constants were obtained by using diffusion-order spectroscopy (DOSY) techniques. 

Nitranions originated by the deprotonation of aldehyde phenylhydrazones can be seen as diazaallylic anions jr-charge delocalization has also been investigated using the NMR approach in these systems112. 

In a different NMR approach, deuterium-labeled p eM o-enantiomeric ketones are reduced to diastereomeric alcohols (theoretically by a hydrogenase), and the product ratio is measured by NMR spectroscopy 100). The method has yet to be tested in enzyme-catalyzed reactions. 

W. Cui, P. Otten, Y. Li, K. Koeneman, J. Yu, R.P. Mason, A novel NMR approach to assessing gene transfection 4-fluoro-2-nitrophenyl-/ -D-galactopyranoside as a prototype reporter molecule for j -galactosidase, Magn. Reson. Med. 51 (2004) 616-620. 

NMR approach for studying Merrifield solid-phase peptide synthesis, let. Lett., 1980, 21, 1397-1400. 

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