Nitrocellulose membrane filters

Place nitrocellulose membrane, filter paper, and fibrous pad in SDS-PAGE transfer buffer for at least 1 h before use. All fibrous pad and filter papers should be totally wet before use. 

Support grid—>fibrous pad—>filter paper—>gel—>nitrocellulose membrane—>filter paper—>fibrous pad—>support grid (see Note 10). 

Transfer membranes. 0 45- um BA 85 nitrocellulose membrane filters (from Schleicher and Schull GmBH, Kassel, Germany) 0 22-pm membranes can be used for low-mol-wt antigenic proteins... 

Filter Preparation. The following protocol for immobilizing single-stranded DNA on nitrocellulose membrane filters is essentially that of Gillespie and Spiegelman.2 Different filter sizes may be used, but it is more efficient to immobilize DNA on large filters which can be subdivided rather than to use many smaller filters. Generally, the amount of DNA immobilized is 25 /ig/cm2. 

Grasshoppers that had been starved for 48 hours were presented with 5 cm2 nitrocellulose membrane filter disks to which 5% sucrose solution had been added. Test disks were treated with 8 8 SC in chloroform solution while control disks received chloroform only. All disks were air-dried. Area of disk eaten was determined with an area meter. 

Nitrocellulose membrane filters (BA83 or BA85) from Schleicher and Schuell, Keene, NH. 

Collection of Fe(II) on a nitrocellulose membrane filter in the form of ion associate with ferrozine and a cation surfactant was applied to the determination of Fe(II) in river and sea water samples [14]. Solid-phase spectrophotometry using ascorbic acid and ferrozine was employed to determine Fe in water, waste water, wine, soil extract, digested vegetal tissues, drugs and human hair [15]. 

Nitrocellulose membrane filters MF-Millipore Membrane Filters, type HA, pore size 0.45 m, diameter 25 mm (Milli-pore, Billerica, MA). 

Nitrocellulose membrane filters are rinsed with 5 mL of YEPD in a vacuum filter manifold. Subsequendy, the mating mixtures are transferred to the membranes by filtration and the membranes rinsed twice with 10 mL YEPD. The filters are then removed with sterile Millipore tweezers (dipped in 70% EtOH and flamed) and placed in 2-mL microcentrifuge tubes containing 2 mL of SC medium. The tubes are sonicated in a sonication bath for 10 s at 8% maximum amplitude (trrNote 8). 

Hincke, M. (1988). Routine Detection of Calcium-binding Proteins Following Their Adsorption to Nitrocellulose Membrane Filters, Anal. Biochem. 170 256-263. 

Nitrocellulose membrane filters, nitrated papers used as membranes in protein engineering and the electrophoresis (electrical separation of particles in a liquid) of DNA and polymeric samples. 

Measurement of dissolved mercury compounds requires removal of particulate materials from the sample by either filtration or centrifugation. Various types of filters can be employed 0.45 pm membrane filters (precipitation, seawater), disposable polystyrene units (Nalgene) with nitrocellulose membrane filters, and pretreated glass fiber filters. 

Nitric Acid, fuming 2032 44 Nitrocellulose Membrane Filters 3270 32... 

It is possible to perform the paired-ion determination without using a water-immiscible solvent. The ion pair formed by an anionic surfactant with many cationic dyes, including methylene blue and rhodamine 6G, is water-insoluble and, with shaking, will adsorb to the walls of the vessel. After discarding the aqueous solution, the ion pair can be washed from the walls with ethanol or methyl cellosolve and measured spectrophotometri-cally. Best results are obtained with a poly(tetrafluoroethylene) vessel (52). In a similar vein, the ion pair can be filtered out, then dissolved and measured spectrophotometrically. This was demonstrated using a cationic iron complex with a nitrocellulose membrane filter which was soluble in the 2-methoxyethanol used for the spectrophotometric measurement (53). 

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