Nickel binding

Haspel, J., F.W. Sunderman Jr., S.M. Hopfer, D.C. Henjum, PW. Brandt-Rauf, I.B. Weinstein, S. Nishimura, Z. Yamaizumi, and M.R. Pincus. 1993. A nickel-binding serpin, pNiXa, induces maturation oiXenopus oocytes and shows synergism with oncogenic ras-p21 protein. Res. Comm. Chem. Pathol. Pharmacol. 79 131-140. 

Nickel binds to multiple high- and low-affinity sites at the DNA molecule. 

Figure 4.1 Nickel transport in . co//.The lower part of the figure shows the nik gene cluster from coli which encodes a nickel specific ABC-type transporter and the repressor protein (NikR).The likely roles of the various nik gene products are indicated in the upper part of the figure.The transporter is encoded by the nikBCDE genes whilst a periplasmic nickel-binding protein is encoded by n/M.The system is expressed when nickel is low. High nickel represses expression via NikR and under these conditions nickel is transported via the Mg transporter (top left).

Rey, L., Imperial, J., Palacios, J.-M. and Ruiz-Argueso, T. (1994) Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis. J. Bacterial., 176, 6066-73. 

Strain HypB expressed Hydrogenase activity (%) Promoter activity (%) Nickel storage GTPase (per mm) Nickel binding... 

In human serum, nickel binds to albumin, L-histidine, and 2 i croglobulin (Sarkar 1984). The principal binding locus of nickel to serum albumin is the histidine residue at the third position from the amino terminus (Hendel and Sunderman 1972). A proposed transport model involves the removal of nickel from albumin to histidine via a ternary complex composed of albumin, nickel, and L-histidine. 

Jack bean urease is a trimer or hexamer of identical 91-kDa subunits while that of the bacterium Klebsiella has an (a(32y2)2 stoichiometry. Nevertheless, the enzymes are homologous and both contain the same binickel catalytic center (Fig. 16-25).435-4373 The three-dimensional structure of the Klebsiella enzyme revealed that the two nickel ions are bridged by a carbamyl group of a carbamylated lysine. Like ribulose bisphos-phate carboxylase (Fig. 13-10), urease also requires C02 for formation of the active enzyme.438 Formation of the metallocenter also requires four additional proteins, including a chaperonin and a nickel-binding protein.438 439... 

Skin sensitization is believed to occur as a result of nickel binding to proteins (particularly on the cell surface) and hapten formation. Essentially, the body perceives the nickel-protein complex as foreign and mounts an immune reaction to it. For example, sweat may react with the nickel in plated jewelry that comes in direct contact with skin dissolved metal may penetrate and react with proteins in the skin and lead to immune sensitization. Nickel may substitute for certain other metals (especially zinc) in metal-dependent enzymes, leading to altered protein function. High nickel content in serum and tissue may interfere with both copper and zinc metabolism. It also readily crosses the cell membrane via calcium channels and competes with calcium for specific receptors. 

This trace element is in combination with iron and selenium in the hydrogenase mentioned previously in the sections on iron and selenium, which is known to be active in oxidation-reduction reactions (giving or taking electrons), an important activity of a class of enzymes. Nickel also has a role in the regulation of hydrogenases. Nickel binds easily to amino acids and to proteins such as globulins (which can be turned into transporters of metals in the bloodstream). Nickel proteins may be used to stabilize certain molecules. 

De Pina K, Navarro C, McWalter L, Boxer DH, Price NC, Kelly SM, Mandrand Berthelot MA and Wu LF (1995) Purification and characterization of the periplasmic nickel-binding protein NikA of Escherichia coli K12. Fur J Biochem 227 857-865. 

Lee MH, Pankratz HS, Wang S, Sgott RA, Ein-negan mg, Johnson MK, Ippolito JA, Christianson DW and Hausinger RP (1993) Purification and characterization of Klebsiella aerogenes UreE protein a nickel-binding protein thatfunc-tions in urease metallocenter assembly. Protein Sd 2 1042-1052. 

Song HK, Muleooney SB, Hubee R and Hau-siNGEE RP (2001) Crystal structure of Klebsiella aerogenes UreE, a nickel-binding metallochaperone for urease activation. J Biol Chem 276 49359-49364. 

Watt RK and Ludden PW (1998) The identification, purification, and characterization of Coo] - A nickel-binding protein that is CO-regulated with the Ni-containing CO dehydrogenase from Rhodospi-rillum rubrum. J Biol Chem 273 10019-10025. 

Templeton DM and Saekae B (1986) Nickel binding to the C-terminal tryptic fragment of a peptide from human kidney. Biochim Biophys Acta 884 382-386. 

Watt RK and Ludden PW (1999) Nickel-binding proteins. Cell Mol Life Sci 56 604—625. 

Complexes 8b" and 8c" ", Figure 8, better mimic the chemical composition of the iron portion of the active site. As a result of the anionic charge, their j/(CX) IR absorptions are much lower than those in the active site. As the anionic charge would be neutralized by nickel binding, an NiFe heterobimetallic should give a more spectroscopically compatible model. Complex 8c was also prepared as the amidosulfido derivative." A six-coordinate complex... 

Watanabe S, Aral T, Matsumi R et al (2009) Crystal structure of HypA, a nickel-binding metallochaperone for [NiFe] hydrogenase maturation. J Mol Biol 394 448 59... 

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