Tryptic fragments

The LC-FTIR part of the study was concerned with the verification of the presence of particular functional groups within each of the tryptic fragments and will not be considered any further here. 

Recently the genome sequence of Methylobacillus flagellatus KT was deposited into the NCBI database and provided a match to our sequence data with a database entry (ZP 00173833). The protein was identified as a hypothetical protein Mflag020121. The sequence of this protein along with the sequences matching the tryptic fragments from our E. sakazakii marker protein (shown underlined) is... 

The equivalent of the tryptic fragment of human transferrin receptor has been expressed in Chinese hamster ovary cells and its structure determined at a resolution of 0.32 nm (Lawrence et ah, 1999). The asymmetric unit of the crystals contains four transferrin receptor dimers. Interpretable electron density is found for the entire tryptic fragment except for Arg-121 at the amino terminus, and density is also seen for the first N-acetylglucosamine residue at each of the N-glycosylation sites. The transferrin receptor monomer is made up of three distinct domains, organized such that the dimer is butterfly shaped (Figure 5.10, Plate 7). The likely orientation of the dimer with respect to the plasma membrane has been assigned on the basis of the... 

Edelman, G.M., Gall, W.E., Waxdal, M.J., and Konigsberg, W.H. (1968) The covalent structure of a human gG-immunoglobulin. I. Isolation and characterization of the whole molecules, the polypeptide chains, and the tryptic fragments. Biochemistry 7, 1950-1958. 

The isolation of NOS led to the cloning and expression of the cDNA for this enzyme. NOS was purified as before and tryptic fragments were isolated. Bredt s group44 then continued to isolate the cDNA by a complex method. The two larger peptides (17 and 18 amino acids) were formed, then a 599-bp product was synthesized from the two of these. This 599-bp product was then used to screen 106 cDNA clones and three clones... 

E. Haas, G. T. Montelione, C. A. McWherter, and H. A. Scheraga, Local structure in a tryptic fragment of performic acid oxidized ribonuclease A corresponding to a proposed polypeptide chain-folding initiation site detected by tyrosine fluorescence lifetime and proton magnetic resonance measurements, Biochemistry 26, 1672-1683 (1987). 

Shape constrained natural compounds (tocopherol homologues, vitamin E)" protein kinase ZAP-70 tryptic fragment containing amino acids 485 96 cartenoids from a spinach sample isoflavines in Radix astragali ... 

Scheme 6 Formation of Two Interchain Disulfide Bridges with Different Connectivities Using Two Kinds of Protecting Groups for the Cysteine Residues. Synthetic Schemes for the Two Isomers of the Tryptic/Chymo-tryptic Fragment of (0-Conotoxin-GVIA,62l...

A tryptic fragment with three interchain disulfide bonds between four peptide chains was synthesized by repetitive application of this Cys activation/disulfide forming method, and by this procedure the disulfide connectivities of synthetic porcine C5a-anaphylatoxin was confirmed to be identical to those for the natural peptide. 67 ... 

Figure 19-15 Ribbon representation of chicken skeletal myosin subfragment-1 showing the major domains and tryptic fragments. Prepared with the program MolScript. From Rayment.157...

Walsh, M., Stevens, F. C., Kuznicki, J., and Drabikowski, W. (1977). Characterization of tryptic fragments obtained from bovine brain protein modulator of cyclic nucleotide phosphodiesterase./. Biol. Chan. 252, 7440-7443. 

Figure S.3 Effect of capillary length on the resolution of tryptic fragments of BSA. Conditions capillary, 375 /xm O.D. X 50 /xm I.D. buffer, 100 mM sodium dihydrogen phosphate, pH 2.5 temperature, 25°C detection, UV absorbance at 200 nm injection, pressure for 10 sec sample, 10/1 dilution of BSA digest. (Reprinted from Ref. 5 with permission.)...

In addition to the attempts at unraveling the modification site, Dive and coworkers also constructed two biotinylated A/BPs, 22 and 23 (Scheme 3c), and used these to study the difference in affinity- and photoaffinity MMP enrichment from a complex proteome [57]. For this, tumor extracts were spiked with hMMP-12 and hMMP-8, after which compounds 22 and 23 were applied, followed by streptavidin-coated magnetic beads for MMP pull-down. Affinity-based labeling with 23 appeared superior to photoaffinity-based labeling with 22 in terms of quantity of captured MMPs and identification of the tryptic fragments by mass spectrometry. 

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