NikA protein

Functional proteins are also involved in high-affinity Ni transport for hydrogenase synthesis. One example is the nikABCDE gene cluster of Escherichia coli 20). NikA is a periplasmic Ni-binding protein. 

De Pina K, Navarro C, McWalter L, Boxer DH, Price NC, Kelly SM, Mandrand Berthelot MA and Wu LF (1995) Purification and characterization of the periplasmic nickel-binding protein NikA of Escherichia coli K12. Fur J Biochem 227 857-865. 

Any commercially available or homemade gel electrophoresis apparatus can be used for the elution-concentration system as long as the dimensions do not significantly vary. Electroblotting is carried out in a Bio-Rad Transblot cell or MiniTrans blot. For details on protein sequencing and HPLC peptide separation, reference is made to the article on amino-terminal protein sequence analysis by Heinz Nika and Ruedi Aebersold in this volume. The scalpels (No. 11) were from Paragon and the long needles (Cat. No. V2A 1415 LL-10) from Acufirm. The power supplies were from Pharmacia-LKB (EPS 500/400). 

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