New Developments in HPLC

and Alsante, K., Handbook of Isolation and Characterization of Impurities in Pharmaceuticab, Academic Press, New York, 2003. 

Chromatography and Separation Science, Academic Press, New York, 2003. 

Chiral Separations by Chromatography, Oxford Press, New York, 2000. 

Impurities Evaluation of Pharmaceuticals, Marcel Dekker, New York, 1998. 

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XXL CHROMATOGRAPHY DATA PROCESSING XXII. NEW DEVELOPMENTS IN HPLC REFERENCES... 

The earlier chapters in this book focus on the current state-of-the-art in HPLC and provide useful practical advice for analyzing pharmaceuticals by HPLC. In this chapter, we plan to offer a glimpse into the new developments in HPLC and discuss how these advances can help improve the performance of HPLC. The progress in HPLC technology has been driven by the need to improve sample throughput because project time lines are short, and the pressure to bring the next blockbuster drug to... 

In this chapter, a few glimpses have been provided into the new developments in HPLC. Many of these advances revolve around reducing not only HPLC cycle times by improving the columns and the instrumentation. 

For reference methods, HPLC with various detectors has become the standard reference technique for analysis of food additives, but new developments in this area are mainly linked to detector technology. Diode array detectors have not totally met the expectations of food analysts in terms of their specificity and LC-MS is likely to fill the gap. Specific detection with biosensor chips may also have a future for certain analyses. The use of combined LC-MS/DAD systems is... 

These interface techniques for connecting the HPLC with the MS-system are very sensitive for most of the substances of interest to the flavour industry. Therefore, HPLC-MS coupling techniques have become an increasingly powerful tool for quality control of flavourings, especially for the analysis of complex mixtures like process flavourings or contaminants present in such complex mixtures. New developments in the area of mass detection systems, such as time-of-flight (ToF) mass analysers and tandem mass spectrometry systems or the features of matrix-assisted laser desorption ionization (MALDI) techniques, may enhance the analytical capabilities of these systems in the near future [16, 17, 28-31 ]. 

High-throughput preparative HPLC coupled to electrospray ionization mass spectrometry (Chapter 17), which disposes upon a signal for collecting detected compounds of the defined molecular mass, is one of the highly promising new developments in this area. Such systems can be incorporated for synthesis purposes into the periphery of automated multicomponent systems, thus making a valuable contribution to the rationalization and quality enhancement of combinatorial synthesis processes. The combination of automated synthesis, purification and on-line instrumental identification (NMR, IR, MS) will become feasible in the near future, and as a matter of routine operation. Analytic methods of structure elucidation will then also be able to be combined with automated combinatorial chemistry. 

There has been an explosion of new developments in mass spectrometry in recent years. One of the major ones is the reduction in size of the instruments so that many can now be used on the benchtop. This has resulted in prices coming down - initial and running costs used to be prohibitive in many cases. The development of atmospheric pressure ionisation sources is relatively new and has made hyphenation of MS with liquid separation techniques such as HPLC much more feasible. The selection of mass analysers... 

In RP-HPLC, the stationary phase is less polar than the mobile phase and is usually comprised of spherical silica particles (typically, 3-5 pm in diameter). The acidic functionalities on the silica material have been modified by deriv-atisation with alkyl (C2 to C18), phenyl, cyano and amino groups. Typical mobile phases used in RP-HPLC consist of mixtures of aqueous buffers mixed with water-miscible organic solvents, such as methanol and acetonitrile. In addition to modified silica stationary phases, other new developments in RP-HPLC are now available, e.g. porous polymeric, carbon and mixed modal phases. 

CEC is a relatively new development in CE that strives to combine the best features of HPLC and CE. Over the last decade, publications in the use of CEC have increased significantly [124-127] signalling the importance of... 

New Developments in Silver Ion and Reverse Phase HPLC of Conjugated Linoleic Acid... 

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