HPLC technologies

Advances in Capillary and Nano HPLC Technology for Drug Discovery and Development... 

Introduces microparallel liquid chromatography (LC), ADME/PK high-throughput assay, MS-based proteomics, and the advances of capillary and nano-HPLC technology... 

HPLC columns only HPLC Technology Wellington House Waterloo St West Macclesfield Cheshire SK11 6PJ UK... 

Another RP-HPLC technology has been developed for the investigation of the carotenoid concentration in the diet, blood and tissues of zebra finch (Taeniopygia guttata). Diet mix (0.5) was pulverized and extracted three times with 2 ml of THF. The... 

Similarly to TLC a high number of HPLC technologies have been developed and employed for the separation and quantitative determination of the biologically active components in medicinal plants and in extracts of medicinal plants. 

A slightly different RP-HPLC technology has been developed and applied for the determination of catechins in tea infusions. Tea infusions were prepared by the traditional method, — filtered and used for HPLC analysis. Separation was carried out in an ODS column... 

This chapter is intended to serve as a general overview of new and emerging HPLC technologies and is divided into four sections simplifying sample preparation, new column technologies, improvements in detectors, and improvements in HPLC throughput. 

The earlier chapters in this book focus on the current state-of-the-art in HPLC and provide useful practical advice for analyzing pharmaceuticals by HPLC. In this chapter, we plan to offer a glimpse into the new developments in HPLC and discuss how these advances can help improve the performance of HPLC. The progress in HPLC technology has been driven by the need to improve sample throughput because project time lines are short, and the pressure to bring the next blockbuster drug to... 

Most of the articles in the scientific literature dealing with analytical methods using HPLC technology for the analysis of aqueous environmental samples employ the reversed-phase mode. This finding is not surprising, as the data in Table V attest to. The flexibility of the reversed-phase mode suits environmental analyses. Many metabolites of environmentally important organic compounds are ionic or ionizable and ideally suited to separation by ion-pair techniques. A variety of... 

In the past 5 years the frequency of reports on the use of HPLC technology for the determination of trace organic compounds in aqueous environmental samples has been steadily increasing. Many innovative approaches to sample cleanup and analyte isolation have been reported. Reversed-phase separation, with its many mobile-phase adaptations, has been and continues to be the most popular HPLC separation mode. The development of fast columns and microbore columns should provide optimal configurations for particular applications. The operating characteristics of microbore columns also make... 

HPLC technology has recently experienced a period of very rapid growth to the extent that, along with GC, conventional LC,... 

Many different products are now purified by chromatographic processes, from the laboratory scale (a few grams) up to the industrial pharmaceutical scale (a few tons per year) or even up to the petrochemical scale (100,000 tons per year). Among the possible technologies, the elution high-performance liquid chromatography (HPLC) technology (sometimes with recycle) has taken a very important part of the small-scale (10 tons per year) market... 

Olsen, B.A., Castle, B.C., Myers, D.P. Advances in HPLC technology for the determination of drug impurities. Trends Anal. Chem. 25, 796-805 (2006)... 

Figure 10.2 Urinary creatinine assay. The chromatographic conditions were as follows column, 150 mm X 4.6 mm (5 pm) cation-exchange silica (SCX HPLC Technology, UKI eluent, 50mM sodium formate adjusted to ca. pH 6-methanol (80 20) flow rate, 1 mimin, injection, 20 pi temperature, ambient detector, variable wavelength UV detector (Cecil) set at 230 nm. Qiroraatogram courtesy Dr Ian James and the author. Department of Medicine St Barts. London.

The separation capabilities of current HPLC technology, as implied by Figure 2, did not arrive by a sudden jump into the "HPLC era". HPLC today is a cumulative advance, a result of progress made by a cast of many thousands who over the years have worked to expand the scope and depth of the field, and who have made their results known to others. 

His company. Waters Associates, had started an applications laboratory in 1967. Woodward s chemical insights were needed, as were Waters technical insights, in order to achieve a proper harnessing of the speed and resolution of the new HPLC technology. It was decided that the problem was to be solved in Woodward s laboratory using the technology that Waters was eager to share. 

Proteomics research is currently driving HPLC technologies towards high sensitivity, speed, and peak capacities.8 Capillary LC (sometime termed micro LC or nano LC)18,19 is used to enhance mass sensitivity needed to analyze minute sample amounts. System requirements for handling extremely low flow rates and small peak volumes mandate the use of specialized HPLC system such as the one shown in Figure 4.14a, which has a micro-pump, a micro x-y-z autosampler, and a PDA detector with a low-volume flow cell. Figure 4.14b illustrates the performance at 12pL/min of this system. 

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