Neuroblastoma cells binding

Graham, D.G. Tiffany, S.M. Bell, W.R., Jr. and Gutknecht, W.F. Autooxidation versus covalent binding of quinones as the mechanism of toxicity of dopamine, 6-hydroxydopamine and related compounds toward C1300 neuroblastoma cells in vitro. Mol Pharmacol 14 644-653, 1978. 

In this sense, we have observed that, unlike tamoxifen, the quaternary derivative ethylbromide tamoxifen fails to block volume-sensitive chloride channels (as those found in lens fibers) in HeLa and Cl300 neuroblastoma cells (unpublished data). Likewise, ethylbromide tamoxifen is totally ineffective on delayed rectifier K+ channels in NG108-15 cells, while tamoxifen is a potent reversible blocker (Allen et al. 2000). From this point of view, nonpermeant SERM derivatives are useful pharmacological tools for investigating whether binding sites in membrane targets are located in the extracellular domains of membrane proteins or, because they can partition into the membrane, interact at some level within the lipid bilayers. 

Although this chapter will only discuss IMS in relation to cell-sorting, this approach has also been adapted for DNA sequencing (1), purification of DNA binding proteins (2), immobilization and isolation of nucleic acids (3), tissuetyping (4,5), quantification of lymphocyte subsets directly from blood (6), bone marrow T-cell depletion (7), depletion of malignant neuroblastoma cells from... 

B. Binding of [125j]Lectins and [125j]Toxin to Neuroblastoma Cells of Primate and Nonprimate Origin. 

Ulex Europeus [ I]Lectin Binding to Neuroblastoma Cell Surfaces... 

Daniel H, Crepel F (2001) Control of Ca(2+) influx by cannabinoid and metabotropic glutamate receptors in rat cerebellar cortex requires K(+) channels. J Physiol 537(Pt 3) 793-800 Davis MI, Ronesi J, Lovinger DM (2003) A predominant role for inhibition of the adenylate cy-clase/protein kinase A pathway in ERK activation by cannabinoid receptor 1 in N1E-115 neuroblastoma cells. J Biol Chem 278(49) 48973-80 Devane WA, Hanus L, Breuer A, Pertwee RG, Stevenson LA, Griffin G, Gibson D, Mandelbaum A, Etinger A, Mechoulam R (1992) Isolation and structure of a brain constituent that binds to the cannabinoid receptor. Science 258 1946-9... 

Chu, W. A., Moehlenkamps, J. D., Bittel, D., Andrews, G. K., and Johnson, J. A. Cadmium-mediated activation of the metal response element in human neuroblastoma cells lacking functional metal response element-binding transcription factor-1. J. Bio. Chem. 274(9), 5279-5284,1999. 

Anandamide parallels d9-THC in competing with the binding of [3H]HU-243 (6) to the brain cannabinoid receptor [33]. However, under most experimental conditions an amidase blocker has to be used in order to prevent anandamide hydrolysis during the binding experiments [38], Using neuroblastoma cells that express this receptor naturally, as well as cultured cell lines transfected with this receptor, it was shown that, like d9-THC, anandamide inhibits A-type voltage-dependent calcium channels [39] and adenylate cyclase [40, 41],... 

Yokosawa, N., Kurokawa, Y., Tsuzuki, K., S mto, B., Fujii, N., Kimura, K., Oguma, K. (1989). Binding of Clostridium botulinum type C neurotoxin to different neuroblastoma cell lines. Infect. Immun. 57 272-7. 

Ca -induced seizures (264). The drug is structurally related to a K-opioid agonist, although it shows no binding affinity to this receptor. Its primary effect is with sodium channels it blocks NlE-115 mouse neuroblastoma cells in a voltage- and use-dependent manner... 

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