Mycobacterium tuberculosis susceptibility testing

M. A. Miller, L. Thibert, F. Desjardins, H. Siddiqi, A. Dascal, Testing the Susceptibility of Mycobacterium tuberculosis to Pyrazinamide Comparision of Bactec Method with Pyrazinamidase Assay , J. Clin. Microbiol. 1995, 33, 2468-2470. 

Materials. The silver composition of the present invention comprised 10 ppm silver in water. The silver composition was evaluated employing a liquid to liquid matrix against Mycobacterium bovis BCG (TMC 1028). This organism causes tuberculosis in animals and can cause tuberculosis in humans. It is used as a stand-in for M. tuberculosis, the major cause of human tuberculosis, as tests have shown it to have a similar susceptibility to M. tuberculosis. The test organism was exposed to the silver composition in duplicate at four exposure times and quantified using membrane filtration. 

Ardito F, Posteraro B, Sanguinetti M, et al. Evaluahon of Bactec Mycobacteria Growth Indicator Tube (MGIT 960) automated system for drug susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol. 2001 38 4440 444. 

Division T.C.T., Tuberculosis Research Centre C., 2009 (Indian C. of M.R., and National Tuberculosis Institute, Bangalore (Directorate General of Health Services G. of I. 2009. Revised National TB Control Programme Training Manual for Mycobacterium Tuberculosis Culture Drug Susceptibility Testing. New Delhi. 

Scarparo C, Ricordi P, Ruggiaro G, Piccoli P. Evaluation of the fully automated BACTEC MGIT 960 system for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide, streptomycin, isoniazid, rifampicin and ethambutol and comparison with the BACTEC 460TB method. J Clin Microbiol 2004 42(3) 1109-14. 

Piersimoni C, Olivieri A, Benacchio L, Scarparo C. Current perspective on drug susceptibility testing of Mycobacterium tuberculosis complex the automated noma-diometric systems. J Clin Microbiol 2006 44 (1) 20-8. 

Banaiee N, January V, Barthus C, Lambrick M, Roditi D, Behr MA, Jacobs Jr. WR, Stein LM. Evaluation of a semiautomated reporter phage assay for susceptibility testing of Mycobacterium tuberculosis isolates in South Africa. Tuberculosis 2008 88(1) 64-8. 

Hillmann D, Gerdes RS, Richter E. Evaluation of Genotype MTBDR Plus assay for rifampicin and isoiuazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens. J Qin Microbiol 2007 45(8) 2635-. ... 

Piuri, M., Jacobs, W.R., Hatfull, G.R, 2009. Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis. PLoS One 4, e4870. 

Isoniazid is the hydrazide of isonicotinic acid. It is a first-line drug for therapy and prophylaxis of tuberculosis. It is bactericidal for rapidly dividing mycobacteria, but bacteriostatic for resting bacilli . Among non-tuberculous mycobacteria, only a few strains, such as Mycobacterium kansasii, are susceptible. As a rule, sensitivity should always be tested in vitro, since the minimum inhibitory concentration varies greatly. 

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