Immunosensors multiplexed detection

This book provides a comprehensive compilation of seven chapters, with important contributions of several authors. Chapter 1 reviews the recent research in using nanoparticle labels and multiplexed detection in protein immunosensors. This chapter summarizes recent progress in development of ultrasensitive electrochemical devices to measure cancer biomarker proteins, with emphasis on the use of nanoparticles and nanostmctured sensors aimed for use in clinical cancer diagnostics. Based on recent strategies focused on nanomaterials for electrochemical biosensors development. Chap. 2 discusses the development of new... 

Viswanathan, S., Rani, C. and Ho, J.A. (2012) Electrochemical immunosensor for multiplexed detection of food-borne pathogens using nanocrystal bioconjugates and MWCNT screen-printed electrode. Talanta, 94, 315-319. 

Neves, M.M.P.S., Gonz ez-Garcta, M.B., Delerue-Matos, C. and Costa-Garcia, A. (2012) Multiplexed electrochemical immunosensor for detection of celiac disease serological markers. Sens. Actuators, B, 187, 33-39. 

Neves M, Gonzalez-Garcia MB, Delerue-Matos C, Costa-Garcia A. Multiplexed electrochemical immunosensor for detection of celiac disease serological markers. Sensors and Actuators B-Chemical 2015 187 33-9. http //dx.doi.Org/10.1016/j.snb.2012.09.019. 

Wu J, Yan Y, Yan P, Ju H (2008) Electric field-driven strategy for multiplexed detection of protein biomarkers using a disposable reagentless electrochemical immunosensor array. Anal Chem 80 6072-6077... 

Gold nanoparticles (Au-NPs) and silver nanoparticles (Ag-NPs) are of particular interest in DNA sensors and immunosensors due to their advantageous properties, such as hydrophilicity, standard fabrication methods, excellent biocompatibility, unique characteristics in the conjugation with biological recognition elements, and multiplex capacity for signal transducer. Therefore, a large number of published methods use Au- or Ag-NPs in DNA [16,17,18] protein [19] and even cell [20] electrochemical detection besides optical detections like ICP-MS [21] or their use as ELISA enhancer [22]. 

Multiplexing has also been achieved with electrochemical immunosensors. Separation of iridium oxide electrodes by 2.5 mm in arrays to eliminate cross-talk enabled simultaneous electrochemical immunoassays using alkaline phosphatase-labeled Ab2 and detection of product hydroquinone giving DLs 1 ng mL for goat IgG, mouse IgG, and cancer biomarkers carcinoembryonic antigen (CEA) and a-fetoprotein (AFP) [35]. An 8-electrode array was developed for detection [36] of... 

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