Multidimensional liquid chromatography tandem mass spectrometry

Mawuenyega, K.G., Kaji, H., Yamuchi, Y., Shinkawa, T., Saito, H., Taoka, M., Takahashi, N., Isobe, T. (2003). Large-scale identification of Caenorhabditis elegans proteins by multidimensional liquid chromatography—tandem mass spectrometry. J. Proteome Res. 2, 23-35. 

N., Isobe, T Yamauchi, T. Molecular constituents of the postsynaptic density fraction revealed by proteomic analysis using multidimensional liquid chromatography-tandem mass spectrometry. J. Neurochem. 2004, 88 759-768. 

Multidimensional liquid chromatography and tandem mass spectrometry... 

One attempt to overcome these disadvantages has been to use multidimensional liquid chromatography (LC) followed directly by tandem mass spectrometry to separate, fragment and identify proteins (Link et al., 1999). In this process, a denatured and reduced protein mixture is digested with a protease to create a collection of peptides (Fig. 2.6). The peptide mixture is applied to a cation exchange column and a fraction of these peptides are eluted based on charge onto a reverse-phase column. The... 

DeSouza, L., Diehl, G., Rodrigues, M.J., Guo, J., Romaschin, A.D., Colgan, T.J., Siu, K.W. (2005). Search for cancer markers from endometrial tissues using differentially labeled tags iTRAQ and cICAT with multidimensional liquid chromatography and tandem mass spectrometry. J. Proteome Res. 4, 377-386. 

Gaucher, S.P., Taylor, S.W., Fahy, E., Zhang, B., Wamock, D.E., Ghosh, S.S., Gibson, B.W. (2004). Expanded coverage of the human heart mitochondrial proteome using multidimensional liquid chromatography coupled with tandem mass spectrometry. J. Proteome Res. 3, 495-505. 

S. McSheehy, J. Szpunar, R. Lobinski, V. Haldys, J. Tortajada, J. S. Edmonds, Characterisation of arsenic species in kidney of the clam Tridacna derasa by multidimensional liquid chromatography-ICP-MS and electrospray time of flight tandem mass spectrometry, Anal. Chem., 74 (2002), 2370-2378. 

Figure 4.8. MuDPIT platform. The MuDPIT or multidimensional gel protein identification system is an multidimensional liquid chromatography (LC)-based system of separation prior to tandem mass spectrometry (MS/MS). It is not necessary to purify proteins prior MuDPIT, although a reduction in protein complexity by some prior purification is helpful in obtaining interpretable spectra. Protein lysates are digested into peptides that are loaded onto a strong cation exchange (SCX) support. Peptides are sequentially eluted onto a reverse-phase column for a second separation. Eluted peptides from the reverse-phase column are electrosprayed into a tandem mass spectrometer for amino acid sequencing and identification of proteins in the sample.

Koch, H. M., Gonzalez-Reche, L. M., and Angerer, J., Online clean-up by multidimensional liquid chromatography-electrospray ionization tandem mass spectrometry for high throughput quantification of primary and secondary phthalate metabolites in human urine, J. Chromatogr. B, 748, 169-182, 2003. 

Lam MPY, Law CH, Quan Q, et al. Fully automatable multidimensional reversed-phase liquid chromatography with online tandem mass spectrometry. In Marlins-de-Souza D editor. Shotgun proteomics methods and protocols, methods in molecular biology. Vol. 1156. New York Springer 2014. p. 39-51. doi 10.1007/978-l-4939-0685-7 3. 

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