MRNA-binding proteins

Runge S, Nielsen FC, Nielsen J, Lykke-Andersen J, Wewer UM, Christiansen J 2000 HI 9 RNA binds four molecules of insulin-like growth factor II mRNA-binding protein. J Biol Chem... 

In addition to bcl-2, another hitherto completely unexpected target for the actions of chronic lithium and VPA has been identified from the mRNA RT-PCR DD study described above. Another clone, also derived from a transcript whose levels were increased by both lithium and VPA, shows very strong homology to a human mRNA-binding protein, the AUH protein ([54, 55] Genbank accession number X79888). BESTFIT analysis revealed 83.2% sequence homology between this rodent clone and the human AUH protein [54—56]. 

Chen G, Manji HK. Mood stabilizers regulate cytoprotective and mRNA binding proteins in the brain Long term effects on cell survival and transcript stability. Int J Neuropsychopharmacol 2001 4 47-64. 

Poly(A)-agarose Nucleic acids containing poly(U) sequences, mRNA-binding proteins... 

Iron regulatory proteins (IRPs) regulate the cellular iron level in mammalian cells. IRPs are known as cytosol mRNA binding proteins which control the stability or the translation rate of mRNAs of iron metabolism-related proteins such as TfR, ferritin, and 5-aminolevulinic acid synthetase in response to the availability of cellular iron [19-21] after uptake [5]. The regulatory mechanism involves the interaction between the iron-responsive element (IRE) in the 3 or 5 untranslated regions of the transcripts and cytosolic IRPs (IRP-1 and -2). IRP-1 is an iron-sulfur (Fe-S) protein with aconitase activity containing a cubane 4Fe-4S cluster. When Fe is replete, IRP-1 prevails in a 4Fe-4S form as a holo-form and is an active cytoplasmic aconitase. As shown in Fig. 3, when Fe is deplete, it readily loses one Fe from the fourth labile Fe in the Fe-S cluster to become a 3Fe-4S cluster and in this state has little enzymatic activity [22, 23]. 

Poly A Sepharose Polyadenylic acid mRNA-binding proteins Viral RNA, RNA polymerase Pharmacia... 

Avoid regions where mRNA-binding proteins may interfere with siRNA binding. 

Prokaryotes do not seem to make extensive use of control at the translational level, whereas eukaryotes use translational control much more widely. In part, translational control in eukaryotes occurs at the mRNA level. It may involve the sequestering of specific mRNAs by combining with specific mRNA-binding proteins and/or rapid degradation of mRNA so that they do not persist in inappropriate phases of the cell cycle. Other translational controls include the phosphorylation of factors involved in translation, as listed below ... 

Proto-oncogene, possible transcription factor DNA-binding protein DNA precursor synthesis, mRNA binding protein Proto-oncogene DNA repair DNA repair... 

TTP is an extraordinarily low-abundance, inducible, stable cytosolic, and hyper-phosphorylated mRNA binding protein [24,25, 31-33]. TTP mRNA and protein... 

Cao H (2004). Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin a zinc-dependent mRNA binding protein affected by posttranslational modifications. Biochem. 43 13724-13738. 

Splicing takes place in a 50-60S small nuclear ribonucleoprotein (snRNP) particle called spliceosome consisting of a pre-mRNA, small nuclear RNAs (snRNAs) and a variety of pre-mRNA binding proteins, which ensures the proper excision of all introns and precise splicing of exons. 

---