Mouse telomerase

Greenberg, R. A., R. C. Allsopp, L. Chin, G. B. Morin, and R. A. DePinho. 1998. Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation. Oncogene 16(13) 1723-30. 

Soder, A., Hoare, S., Muire, S., Balmain, A., Parkinson, E., and Keith, W. (1997) Mapping of the gene for the mouse telomerase RNA component, Terc, to chromosome 3 by fluorescence in situ hybridization and mouse chromosome painting. Genomics 41, 293-294. 

For ease of comparison, the percentage attachment at 15 mins was taken, and a comparison drawn between Human primary skin fibroblasts at 14 and 31 PDs, the telomerase transfected human fibroblasts at 44 PDs, and a commercially available mouse immortal fibroblast line, 3T3 cells (Figure 3). 

Telomerase activity can also be inhibited by the direct binding of small non-nucleosidic synthetic compounds to the hTERT reverse transcriptase component of telomerase. Schnapp and coworkers have recently reported the first mixed-type noncompetitive (70) catalytic telomerase inhibitor, (2-((E)-3-naphtalen-2-yl-but-2-enoylamino)-benzoic acid) (BIBR1532), which causes telomere shortening and senescence characteristics in various types of cancer cells in vitro and in vivo in mouse xenograft models at nanomolar concentrations (71). 

The absence of PARP-2 in PARP-2 Mouse embryonic fibroblasts (MEFs) cells has no effect on mean telomere length measured by Q-FISH. Telomerase activity is also unpermrbed in these cells compared to wild type cells. However, there is a s nificant spontaneous increase in the signal-free ends in PARP-2 cells (Fig. 9B), as well as in the heten eneity of telomere lengths per chromosome, in addition to an increase in the fi equency of spontaneous chromosomes and chromatid breaks. These observations are suggestive of a telomere dysfunction in the absence of PARP-2. 

Park, K. D., Seong, S. K., Park, Y. M., Choi, Y, Park, J. H., Lee, S. H., Baek, D. H. et al. 2011. Telomerase reverse transcriptase (TERT) Related with Telomerase Activity Regulates Tumorigenic Potential of Mouse Embryonic Stem Cells. Stem Cells Dev, 20 149-57. 

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