Chromosome painting

Milan, D., Yerle, M., Schmitz, A., Chaput, B., Vaiman, M., Frelat, G., and Gellin, J. (1993) A PCR-based method to amplify DNA with random primers determining the chromosomal content of porcine flow-karyotype peaks by chromosome painting. Cytogenet. Cell Genet. 62,139-141. 

Total chromosome painting probes, each specific for a different pair of homologs... 

LLNL chromosome paints commercialized. See http /Aiu w.nmi. go /ftgrnis/publicat/4ign/ -7n5A)i coUab.html Human Genome News, 7 5h t (January-March 1996) Collaborations Multiply Research. Commercial Benefits."... 

For numerical chromosomal alterations, it is possible to analyze interphase cells using FISH with whole chromosome paints, or chromosomal specific probes. Thus, it is feasible to assess nondisjunctional events in any cell type, including target cells for tumorigenesis. Other fluorescence-based methods described in the section on in vitro methods (Section 25.3.4b) can be used in vivo, but they are of rather limited value for basic mutagenicity assays. 

So-called chromosome painting refers to the hybridisation of fluorescently labelled chromosome-specific probes (Figure 19.3). Chromosome painting allows the visualisation of individual chromosomes in metaphase or interphase cells, and the identification of both... 

Figure 19.3 Human chromosomes painted by fluorescent dyes to detect abnormal exchange of genetic material frequently present in cancer (note exchange between pink - P and light blue - B chromosomes). Source US Department of Energy Genome Programs (http //genomics.energy.gov).

Tucker JD, Lee DA, Ramsey MJ, et al. (1994) On the frequency of chromosome exchanges in a control population measured by chromosome painting. Mutation Research 313 193-202. 

Chromosome Paints Whole chromosomes Tranlocations Chromosome identification Easy to use High... 

Soder, A., Hoare, S., Muire, S., Balmain, A., Parkinson, E., and Keith, W. (1997) Mapping of the gene for the mouse telomerase RNA component, Terc, to chromosome 3 by fluorescence in situ hybridization and mouse chromosome painting. Genomics 41, 293-294. 

Confirmation of chromosomal identification is obtained by co-hybridizing the test cell line DNA with a whole chromosome paint and visualizing the two probes with different fluorochromes. We have found this to be an important modification to standard REVISH protocols because it allows unambiguous chromosome identification in laboratories without cytogenetic experience. 

Probe Denaturation Double Hybridization of Genomic DNA and a Chromosome Paint... 

Commercial digoxigenin labeled chromosome paint is ready, but not denatured. 

You are now ready to denature both the chromosome paint and your test genomic DNA sample. Both the paint and genomic probe require an incubation at 37°C to allow for suppression of repetitive sequences in the probe by cotl DNA in the hybridization mix. The exact conditions for the chromosome paint denaturation and preannealing will vary with the commercial source of the paint. You will therefore want to plan the next stage to ensure that both the probes complete the preannealing step at the same time. Refer to Fig. 1 for an overview of timing. 

Denature Chromosome Paint According to Manufacturers Instructions For Oncor biotin labeled chromosome paints ... 

Joos, S Scherthan, H., Speicher, M. R., Schlegel, J Cremer, T and Lichter, P. (1993) Detection of amplified DNA sequences by reverse chromosome painting using genomic tumor DNA as probe. Human Genet. 90, 584-589. 

Zhang LS, Honma M, Matsuoka A, Suzuki T, Sofuni T et al (1996) Chromosome painting... 

Kleinerman, R. A., Romanyukha, A. A., Schauer, D. A., and Tucker, J. D. (2006). Retrospective assessment of radiation exposure using biological dosimetry Chromosome painting, electron paramagnetic resonance and the glycophorin a mutation assay. Radiat Res 166, 287-302. 

During Metaphase, Chromosomes Can Be Distinguished by Banding Patterns and Chromosome Painting... 

Banding analysis and chromosome painting, a more precise method, are used to identify the different human metaphase chromosomes and to detect translocations and deletions (see Figure 10-29). 

Metaphase chromosomes can be Identified by characteristic banding patterns. What are G bands and R bands What is chromosome painting, and how Is this technique useful ... 

Figure 7. Occurence of chromatid breaks in centromeric r on 7 h after 2 Gy irradiation. Metaphasespreads of bone marrow cells from irradiated PARP-2 (panel A) and PARP-2 mice (panel B-Q. Arrows point to absence of centromeric signals. Giemsa staining (A,B) Chromosome painting with a centromeric probe, counterstaining with DAPI (C). (Taken from M6iissier-de Murcia et al, with permission). A color version of this figure is available online at www.Eurekah.com.

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