Mouse light chain loci

Carson, S. Wu, G.E. (1989). A linkage map of the mouse immunoglobulin lambda light chain locus. Immunogenetics 29, 173-179. 

Schupp, I.W., Schlake, T., Kirschbaum, T., Zachau, H.G., Boehm, T. (1997). A yeast artificial chromosome contig spanning the mouse immunoglobulin kappa light chain locus. Immunogenet-ics 45, 180-187. 

Using mouse-mouse hybrids and Robertsonian translocation markers, Hengart-ner et al. [86] confirmed that the expression of k light chains requires a locus on chromosome 6, whereas the expression of heavy chains required chromosome 12. The formal demonstration that the structural k and Igh loci reside on chromosomes 6 and 12, respectively, was obtained by the analysis of mouse-hamster hybrids using Southern blot hybridization [87,88], A similar approach was used by D Eustachio et al. [89] to assign the A locus to mouse chromosome 16. 

No enhancer has yet been found in the mouse A locus, although transfection experiments indicate that expression of a transfected, rearranged A, light chain gene in certain plasmacytoma lines manifests an enhancer requirement (unpublished observations). However, the mouse A locus possesses only two VA genes and, therefore, there may be no requirement to place an enhancer downstream of the... 

The IgH locus encodes the antibody heavy chain, and the IgK and IgA, loci encode the light chains. Two Ig-deficient mice lines were constructed by inactivation of the murine IgH or the IgK loci using homologous recombination. Cross-breeding of the two mouse lines resulted in a double inactive mouse strain. 

Light chains controlled by the c locus appear, from a comparison with human and mouse L chains, to be of the X type (71-73). The evidence includes the presence of a blocked N-terminal group (pyrrolidone-carboxylic acid), the C-terminal dipeptide, -Cys-Ser, characteristic of k chains, and the sequence of eight amino acids at the C-terminus (Table 9.2). 

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