Antibodies Heavy chain

This chapter describes the construction of an Fab sequence bearing a foreign gene on the truncated antibody heavy chain, and gives a typical protocol for expression of the fused protein in Escherichia coli. 

Tumas-Brundage, K. Manser, T. (1997). The transcriptional promoter regulates hypermutation of the antibody heavy chain locus. J. Exp. Med. 185, 239-250. 

Fig. 2. Effect of exogenous gelatin [46] and PVP [47] on the production and stability of a murine antibody heavy chain. Triangular symbols indicate PVP, diamonds indicate gelatin, and circles indicate no stabilizer (control). In the upper portion of the figure, the intracellular protein is unaffected by the addition of exogenous stabilizers. In the lower portion of the figure, added PVP and gelatin significantly increase the level of extracelluar protein product...

Reactors of this type have been used to produce and harvest the heavy chain of a murine monoclonal antibody using protein G affinity and histidine-tagged human granulocyte-macrophage colony-stimulating factor (GM-CSF) using metal affinity chromatography. Implementation of this scheme on a small scale was difficult, but these studies show that the amount of recoverable protein can be increased [62]. As shown in Fig. 4, recoveries of human GM-CSF and a mouse monoclonal antibody heavy chain were increased by three- and seven-fold, re-... 

Fc receptor Present on some cells including macrophages and is the receptor to which the constant region of antibody heavy chains (the Fc region) attaches. 

Vh variable region of antibody heavy chain, virology The study of viruses, viscera The collective term for the internal organs, visceral afferent fibres Nerve fibres that convey sensory information to the spinal cord, visceral efferent fibres Nerve flbres of the sympathetic and paia mpathetic nervous systems which convery information from the CNS to the periphery, e.g. to glands, smooth muscle. 

A FIGURE 23-19 Chromosomal translocation in Burkitt s lymphoma. As a result of a translocation between chromosomes 8 and 14, the c-myc gene is placed adjacent to the gene for part of the antibody heavy chain (Ch), leading to overproduction of the Myc transcription factor in lymphocytes and hence their growth into a lymphoma. 

The IgH locus encodes the antibody heavy chain, and the IgK and IgA, loci encode the light chains. Two Ig-deficient mice lines were constructed by inactivation of the murine IgH or the IgK loci using homologous recombination. Cross-breeding of the two mouse lines resulted in a double inactive mouse strain. 

Practically, one often compares the conformation of a protein under two or more conditions. In that case, the difference of the protection factors for the two or more conditions is also minimized during the model optimization. As a result, the difference in protection factors of the two conditions only becomes visible when the differences are statistically significant (Figure 7.7). Taking antistreptavidin lgG2 as an example, the regions of the antibody heavy chain that are destabilized (decreased protection factors) by reduction of interchain disulfide bonds are clearly visible in the differential protection factor plot shown in Figure 7.7b. 

JV-2,4-DinitrophenyI- alanine Anti-dinitrophenyl antibody Heavy chain labeled 36... 

N-t- (4-Azido-2-nitro-phenyl)lysine Binding site of anti-dinitrophenyl antibody Heavy chain labeled between 29 and 34 and 95 and 114 (i.e., two of the hypervariable regions) 107... 

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