Lambda light chains

Immunohistochemical stains for kappa and lambda light chains were performed in the bone marrow specimens to determine the presence or absence of light chain preponderance or monoclonality. In all these cases, including those... 

The specific labeled separated protein fractions blotted on a nitrocellulose membrane or specific immunoflxation-separated protein fractions in polyacrylamide after isoelectric focusing make it possible to detect some additional bands in CSF, i.e., IgM, IgA, free kappa or lambda light chains of specific antibodies (i.e., antiherpes, anti-borrelia, or anti-HIV) (LI, M3). 

Carson, S. Wu, G.E. (1989). A linkage map of the mouse immunoglobulin lambda light chain locus. Immunogenetics 29, 173-179. 

Kindt, T.J., Gris, C., Guenet, J.L., Bonhomme, F., Cazenave, P.-A. (1985). Lambda light chain constant and variable gene complements in wild-derived inbred mouse strains. Eur. J. Immunol. 15,... 

Weigert, M.G., Cesari, I.M., Yonkovich, S.J., Cohn, M. (1970). Variability in the lambda light chain sequences of mouse antibody. Nature 228, 1045-1047. 

Figure 1. Sequential double staining method performed with the EnVision G 2 Doublestain Kit using polyclonal anti-kappa light chains (red) and polyclonal anti-lambda light chains (brown) as primary antibodies. Formalin-fixed, paraffin-embedded tissue sections from tonsils.

FIGURE 6.4 Lambda (A) and kappa (B) immunoglobulin light chain antibodies demonstrate the clear monotypia of the plasma cells in this multiple myeloma. Kappa light chain mRNA in situ hybrization (C) and lambda light chain mRNA (D). 

The detection of kappa or lambda messenger RNA via an in situ hybridization reaction provides an alternative to the immunohistochemical detection of cytoplasmic kappa or lambda light chain (Fig. 6.4C and D). This technique allows for a much cleaner reaction product because it is only demonstrated where there is mRNA for kappa and lambda and not protein. Several immunohistochemical standards now provide an automated platform for the performance of this in situ hybridization reaction. This allows for this technique to enter routine performance and can be accomplished in approximately 5 hours. 

FIGURE 11.29 Monotypic labeling for lambda light-chain immunoglobulin (A) and negativity for kappa light-chain (B) in primary plasmacytoma (C) of the mediastinum. 

Magro CM, Crowson AN, Porcu P, et al. Automated kappa and lambda light chain mRNA expression for the assessment of B-cell clonality in cutaneous B-cell infiltrates its utility and diagnostic application. J Cutan Pathol. 2003 30 504-511. 

Assessment of the transcription type in the cells of interest, this is commonly used to detect kappa and lambda light chains as an important criterion for the diagnosis of lymphoid neoplasia, mainly plasma cell neoplasia. 

BIOMED-2 primers for the detection of the lambda light chain (Ig X) gene rearrangement. The multiplex reaction is carried out with both V X foreword primers (FR) and the J X reverse primer (RP) ... 

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