Molecular genetics cloning

Muller, J., Dijl, J.V., Venema, G. and Bron, S. (1996) Cloning of heterologous genes specifying detrimental proteins on pUC-derived plasmids in Escherichia coli. Molecular Genetics and Genomics, 252, 207-211. 

Molecular genetic techniques have confirmed the existence of multiple subtypes of p-adrenoceptors. Pi-Receptors and Pj-receptors have been cloned, and recent molecular biological evidence indicates the existence of at least one additional p-receptor sub-type, called the p3-receptor. It is suggested that the P3-receptor may mediate some of the metabolic effects of catecholamines, although no available p-blocker has been shown to rely on Pa-receptor antagonism for its therapeutic effectiveness. 

The preferred modem procedure for investigating an enzyme mechanism is to identify a protein in a genome sequence, clone and express it, solve the crystal and/or solution structures, guess the mechanism, and then confirm it by site-directed mutagenesis and kinetics. The mechanism of lysozyme almost illustrates that procedure. Although the classical protein chemistry on lysozyme predated the molecular genetics, the kinetic studies were stimulated by the x-ray crystallography, and the previously unknown mechanism almost leapt from the... 

Skjot, M., Kauppinen, S., Kofod, L. V., Fuglsang, C., Pauly, M., et al., Functional cloning of an endo-arabi-nanase from Aspergillus aculeatus and its heterologous expression in A-oryzae and tobacco. Molecular Genetics Genomics 2001, 265 (5), 913-921. 

B, Nilsson SB, Bennett EP, Jersild C, Clausen H. Cloning and 57. expression of the histo-blood group Pk UDP-galactose Galbeta l Glcbeta 1-Cer alpha 1, 4-galactosyltransferase. Molecular genetic basis of the p phenotype. J. Biol. Chem. 2000 275 16723- 58. 

Bioinformatics/Molecular Genetics Full-length Cloning... 

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