Conventional molecular cloning

Given the organization of the fish taste system, which is comparable with that of mammals, it is natural to assume a parallelism among them in terms of signal transduction mechanisms. Conventional molecular cloning approaches were followed... 

Once the plasmid has been created using conventional molecular cloning methods (Green Sambrook, 2012), it can be amplified by transforming competent Escherichia coli. 

Until recently, receptors were hypothetical macromolecules whose existence was postulated on the basis of pharmacological experiments. Although recent advances in molecular biology have led to cloning and expression of maity of those receptors whose existence was postulated as well as a plethora of subtypes, progress in most cases in defining their three-dimensional structure has yet to provide the medicinal chemist with the necessary atomic detail to design novel compounds. Without detailed information about the three-dimensional nature of the receptor, conventional computationally based approaches, such as... 

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