MilliQ® water

Sodium formate (anhydrous) [141-53-7J M 68.0, m 253°, d 1.92. A saturated aqueous solution at 90° (0.8mL water/g) was filtered and allowed to cool slowly. (The final temperature was above 30° to prevent formation of the hydrate.) After two such crystns the crystals were dried in an oven at 130°, then under high vacuum. [Westrum, Chang and Levitin J Phys Chem 64 1553 I960-, Roecker and Meyer J Am Chem Soc 108 4066 1986.] The salt has also been recrystd twice from ImM DTPA (diethylenetriaminepentaacetic acid which was recrystd 4x from MilliQ water and dried in a vac), then twice from water [Bielski and Thomas J Am Chem Soc 109 7761 1987]. 

Pharmaceutical grade ibuprofen, pseudoephedrine HCl, chlorpheniramine maleate, nipagen and syrup preparation were kindly supplied by Berko tlag Sanayi A. , Istanbul. 0.03 M H PO and KH PO solutions were prepared with analytical grade chemicals and milliQ water. HPLC grade acetoiutrile was used. 

The reaction was quenched by transferring it into a 50 mL Falcon tube containing 33 mL of ice-cold acidic methanol and left immersed in ice for 20 min. The proteinaceous pellet was precipitated by centrifugation at 8000 rpm for 15 min at 4 °C and the supernatant collected in a glass bottle. The pellet was re-extracted twice by washing it in MilliQ water (5 mL) and reprecipitated with ice-cold 100% methanol (35 mL) over 20 min in ice and then centrifuged as above. The combined supernatants were evaporated at 35 °C and the residue was reconstituted in 20 mL of mobile phase (4—8 % MeCN in 5 him ammonium acetate, pH 5.0). 

Nanoprecipitation was carried out according to the procedure described by Stainmesse et al. (14) (Fig. 2). AmB was dissolved in methanol (10 mL), usually at a concentration of 0.35mg/mL (366 pM). This solution was mixed with a solution of phospholipids at different concentrations (typically 1 mg/mL, 1.3 mM), also in methanol (5mL), at 40°C. This organic phase (15mL) was then added to a water phase (MilliQ water, 15 mL, Waters Millipore, France) under gentle magnetic stirring at 20°C. The methanol was then removed by low-pressure solvent evaporation to yield an aqueous dispersion in a volume of 15 mL. 

The Au-coated slide is washed with PBS 0.05% Tween 20 (PBST) 5 times and with MilliQ water 5 times and dried with nitrogen gas flow. 

Typically, the anionic surfactant SDS (65 mg) was dissolved in milliQ water (3 mL), followed by catalyst 1 (13.8 mg, 0.0075 mmol). Stirring was performed at 700 rpm. Constant temperature (25 °C) was maintained by water circulation through an external jacket connected with a thermostat. 

Therefore, an effective water system is required. Nowadays, several techniques can be used to obtain water of high pharmaceutical quality. These include ionexchange treatment, reverse osmosis, distillation, electrodialysis, and ultrafiltration. However, there is no single optimum system for producing high-purity water, and selection of the final system is dependent on factors such as the quality of raw water, intent of its use, flow rate, and costs. In the pharmaceutical industry, the different water classes normally encountered are well water, potable water, purified water, and specially purified grades of water, such as water for injection (e.g., MilliQ water). 

MilliQ water obtained by passing deionized water through a MilliQ Labo system (Millipore). 

Saponification reagent dissolve 1 g KOH in 5 ml MilliQ water and add 45 ml ethanol to make a 0.3 N KOH/ethyl alcohol solution. 

Lin and Hsu [1.85] described the determination of residual moisture in protein pharmaceuticals in sealed glass vials by near-infrared (NIR) spectroscopy. Five proteins were studied recombinant humanized monoclonal antibody (rhuMAb) E25, rhuM-Ab HER2, rhuMAb CDlla, TNKase and rt-PA. Higher moisture contents (RM) were obtained by adding appropriate amounts of MilliQ water to the wall of the vial in a... 

MilliQ water (ddH20) and RNase free water Sodium phosphate (mono- and dibasic)... 

Reconstitute the fractions in MilliQ water (Millipore) and keep them at 4°C for immediate biological activity screening (see Note 9) or in the freezer until use. 

Stock solution of each of the proline-rich peptides (PRP) to be tested in MilliQ water slightly acidified with acetic acid (peptide concentration from 1 to lOmg/mL). 

Agar medium (see 2.1) containing lOOpg/mL ampicillin (ampicillin stock solution lOOmg/mL in MilliQ water, store at —20°C). 

Trypan Blue (Sigma-Aldrich), stock solution of 5 mg/mL in MilliQ water filtered using a 0.22 pm membrane filter (Millipore). 

Several publications have addressed multielemental analysis in milk whey, cow s milk, human milk and infant formulas using SF-ICP-MS (see Table 13.9). Rivero Martino et al. [96] reported the multielemental analysis of a wide range of elements in human, cow s, and formula milk whey samples. Milk whey was obtained by centrifugation and then diluted 1 4 (v/v) with milliQ water for minor and trace elemental analysis (Al, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sr, and Zn) and 1 1999 (v/v) for major elements (Ca, Mg, and Na). Gallium, Rh,... 

Figure 4.1. Measurement of the Pb concentration of laboratory water produced by a reverse-osmosis/ MilliQ water system. The concentration, given by the gradient of this line, was 0.16 pg/g (27).

Chemicals should be analytical grade or the purest grade commercially available. All aqueous solutions should be prepared in sterile, distilled, deionized, ultrafiltered water (prepared using a MilliQ water purification unit [Millipore, Bedford, MA] or similar apparatus). Solutions and reagents are stable at room temperature unless specifically stated otherwise. All equipment should be sterile or sterilized before use. 

Fig. 25 Swollen thickness of PMAA brushes as a function of the external salt concentration for aqueous sodium, calcium, and aluminium nitrate solutions. Values at a concentration of 10 6 mol L 1 correspond to the swelling in pure MilliQ water. The dry film thicknesses were 42 nm (stars), 45 nm (circles) and 46 nm (squares)...

Experiments were carried out on new precipitates in the NaCl electrolyte. All solutions were of analytic grade, prepared with milliQ water quality and bubbled by Na during one night before and during the experiment. 

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