MILLIPORE CORP

Poly(diallydimethylammonium chloride) (PDDA) was purchased from Aldrich Chemical Co. 2-Morpholinoethanesulfonic acid (MES) was from Wako Pure Chemical Industries Ltd. Poly(butylviologen) (PBV), shown in Scheme 1, was synthesized according to literature method and checked by HNMR [7], Ultrapure water (18.3 M l) was prepared with a Milli-Q filtration unit of Millipore Corp. 

Another way to detect small molecules in the final formulated protein product without the interference from the protein signals is to remove the protein by ultrafiltration. Figure 12.4 compares a section of the proton NMR spectra of a biopharmaceutical protein product before (upper spectrum) and after (bottom spectrum) the protein was removed by ultrafiltering the sample with a Centricon-10 (Millipore Corp, Bedford, MA). Removing protein results in a flatter baseline (bottom spectrum). If small molecules are present in a protein sample, the removal of the protein may allow for unobstructed detection of the small molecules. In this case, a small amount of acetate ( 1 pg/rnl) is detected in the sample [bottom trace, Figure 12.4], Figure 12.5 shows that spikes of 10 p.g/ml of acetate and MES into the protein sample are fully recovered after the ultrafiltration to remove the protein. This example demonstrates that the interference of protein with the detection and quantitation of small-molecule impurities in a formulated protein product can be effectively eliminated by ultrafiltration. 

Onofrey, T. and Kazan, G. Performance and Correlation of a 96-Well High throughput Screening Method to Determine Aqueous Drug Solubility, Application Note (Billerica, MA Millipore Corp., 2003). 

All solutions were filtered through a 0.5 ym Fluoropore poly(tetrafluorethylene) filter (FHUP, Millipore Corp.). The solvents trichlorobenzene (TCB), o-dichlorobenzene (ODCB) and a-chloronapthalene (aCN) were used as received without further purification. 

Refractive Index Detector (Knauer). The KMX-6 scattering intensity was measured with the 6-7 degree forward-scattering annulus. A series of Zorbax PSM columns (DuPont) was used PSM 60, PSM 1000, PSM 1000, PSM 60, PSM 1000. Tetrahydrofuran (THF) from Baker was filtered through a 0.22 micrometer Fluoropore filter (Millipore Corp.) before use in chromatography, and a flow rate of 0.7 ml/min was used. 

Fig, 3. Scanning electron microscopic (SEM) pictures of (a) fumed silica and (b) colloidal silica. (Courtesy of Millipore Corp., Bedford, MA.)... 

Water was purified by using a Milli-RO-4 and a Milli-Q water purification system (Millipore Corp., Bedfort, MA 01730, USA). Unless otherwise stated Milli-Q-water quality was used. 

PVDF membrane (e.g, Immobilon PTM from Millipore Corp, Bedford, MA)... 

HPLC Grade n-butanol (Mallinckrodt, Paris KY), and Milli-Q water (Millipore Corp., Bedford MA) were used for all experiments. 

Milli-Q water Reverse osmosis water which is passed through a Milli-Q Plus system (Millipore Corp.) to produce water, which meets the American Society of Testing Materials (ASTM) type 1 reagent grade water standard. Volume 1(6). 

Millipore Corp. technical publications http //www.millipore.com/ techpublications/techl/5f5nrn. 

Millipore Corp. Waters Chrom. Div., 34 Maple St., Milford, MA SPE, dilution, filtration, HPLC injection, mixing Indirect/direct PC/menu based Serial or batch Discontinued... 

Initially, plasma and oral fluid specimens from patients (n = 21) on different antidepressant treatment were collected twice to assess if any of the studied analytes was likely to show a good correlation. The best results were obtained for venlafaxine (%CV for plasma/oral fluid concentrations ratio (f OF/PL) <21%). Therefore, the study was extended for this antidepressant by analysis of oral fluid and plasma specimens from five patients on venlafaxine treatment collected on four occasions. Daily doses of venlafaxine retard formulations were 75 mg for two patients, and 150 mg for the remaining participants. Collection of oral fluid (direct spitting into polypropylene tubes) and plasma (heparinized tubes) specimens was performed, when possible, before the next dose to ensure the drug was in the elimination phase. The dose and the time of collection was the same on the four different occasions for each patient. For the analysis, oral fluid and plasma specimens were centrifuged at 14 x 103 rpm, and 0.2 mL of the supernatant were extracted. In addition, correlation between the concentrations in the plasmatic free fraction and in oral fluid was also evaluated. Plasmatic proteins were eliminated by filtering 0.5 mL of plasma samples using Microcon filter devices Ultracel YM-3 (Millipore Corp., Billerica, MA, USA). 

Soot from an 02-propane flame was produced in a 5 cm diameter 1-meter flow tube, with a total flow rate (N2 carrier gas) of 3.5 liter min. 1 0.2 pm pore size Fluoropore filters (Millipore Corp.) were used for mass evaluation. 

The DynacelT culture system (Millipore corp Appendix 3) allows medium constituents to diffuse through a 0.6/xm membrane into a thin cell growth compartment particuarly suitable for growth of suspension cells. Several units can be coupled together to run from the same perfusing bottle. 

For small volumes, 13 or 25 mm diameter, filtration membranes may be fitted into plastic or stainless steel holders (e.g. the Swinnex filter holder made by the Millipore Corp. Appendix 3) which, after autoclaving, are fitted onto a syringe containing the liquid to be sterilised. Care must be taken in the assembling of the membrane in the holder as incorrect assembly leads to the escape of the membrane from its retaining gaskets with subsequent failure of the filtration process. The plastic holders have a limited life time as they distort on autoclaving. 

When applied to suspension cells the acid washing steps may be done by repeated centrifugation but this is tedious and leads to losses of cellular material unless care is taken. Alternatives involve retaining the cells on glass fibre or cellulose acetate filters in a microanalysis filter holder (Millipore Corp. Ltd.) or the cells may... 

GS-filter 0.22 fim membrane filter supplied by Millipore Corp... 

Figure 9.16. Separation of a mixture of antihistamines and decongestants by ion pair chromatography. Courtesy of Millipore Corp., Waters Chromatography Division.

MultiScreen Filter Assembly with Ultracel-PPB Membrane No. MAPPB1010 (Millipore Corp -Bedford MA USA)... 

ScienCell Research Laboratories 3H Biomedical AB Millipore Corp. 

ACS Specifications similar to CAP Type II. NCCLS specifications similar to CAP. Source of information Millipore Corp. 

All carbons used in this research were pretreated to remove fines by sonication (Bransonic 220, Branson Cleaning Equipment) for 30 seconds followed by a rinse with Type I reagent-grade water (Millipore Corp). Coal-based carbons were then extracted with 2-N HCl in a soxhlet extractor for 42 hours to remove ash components and alkaline impurities. After extraction, the sample was boiled for four hours, then exhaustively rinsed until the pH of the suspension was in the range 5 to 6. All carbon samples were dried at 100 °C under vacuum, and stored in a vacuum desiccator. 

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