Microchip electrophoresis amperometric detection

Cheng, H., Huang, W.-H., Chen, R.-S. et al. 2007. Carbon fiber nanoelectrodes apphed to microchip electrophoresis amperometric detection of neurotransmitter dopamine in rat pheochromocytoma (PC 12) cells. Electrophoresis 28 1579-1586. 

C. -C. Wu, R.-G. Wu, H.-G. Huang, Y.-C. Lin and H.-C. Chang, Three-electrode electrochemical detector and platinum film decoupler integrated with a capillary electrophoresis microchip for amperometric detection., Anal. Chem., 75 (2003) 947-952. 

Three-electrode electrochemical detector and platinum film decoupler integrated with a capillary electrophoresis microchip fin amperometric detection. Anal Chem 75 947-952... 

Fischer J, Barek J, Wang J (2006) Separation and detection of nitrophenols at capillary electrophoresis microchips with amperometric detection. Electroanalysis 18 195-199... 

Wang, J. and M. Pumera. Dual conductivity/amperometric detection system for microchip capillary electrophoresis. Anal. Chem. 74, 5919-5923 (2002). 

The aim of this chapter is to show through the subsequent sections the present trends in capillary electrophoresis microchips paying special attention to the manufacturing and designs employed in those combined to electrochemical (EC) detection. Conductimetric and amperometric detection are considered. Clinical application of these devices is further revised. 

W.R. Vandaveer IV, S.A. Pasas, R.S. Martin and S.M. Lunte, Recent developments in amperometric detection for microchip capillary electrophoresis, Electrophoresis, 23 (2002) 3667-3677. 

J. Vickers and C.S. Henry, Simplified current decoupler for microchip capillary electrophoresis with electrochemical and pulsed amperometric detection, Electrophoresis, 26 (2005) 4641-4647. 

D. J. Fischer, W.R. Vandaveer IV, R.J. Grigsby and S.M. Lunte, Pyro-lyzed photoresist carbon electrodes for microchip electrophoresis with dual-electrode amperometric detection, Electroanalysis, 17 (2005) 1153-1159. 

C.D. Garcia and C.S. Henry, Direct determination of carbohydrates, amino acids, and antibiotics by microchip electrophoresis with pulsed amperometric detection, Anal. Chem., 75 (2003) 4778-4783. 

J. Wang, S. Mannino, C. Camera, M.P. Chatrathi, M. Scampicchio and J. Zima, Microchip capillary electrophoresis with amperometric detection for rapid separation and detection of seleno amino acids, J. Chromatogr. A, 1091 (2005) 118-177. 

Separation and amperometric detection of hydrogen peroxide and L-ascorbic acid using capillary electrophoresis microchips... 

Fu, C. G., Fang, Z. L., Combination of flow injection with capillary electrophoresis Part 7. Microchip capillary electrophoresis system with flow injection sample introduction and amperometric detection. Anal. Chim. Actfl 2000,422(1), 71-79. 

FIGURE 41.4 Examples of electrophoresis microchip fabricated in PT. (A) Microdevice with cupper tape electrodes (a) positioned externally over the channel for C D measurements. Point b is a representation for the solution reservoirs. (Reprinted from do Lago, C.L., Silva, H.D.T., Neves, C.A., Brito-Neto, J.G.A. and Fracassi da Silva, J.A., Anal. Chem., 75, 3853, 2003. With permission.) (B) Microdevice layout for end-channel amperometric detection. S, SW, and b represent, sample, sample waste, and buffer reservoirs, respectively. (Reprinted from do Lago, C.L., et al.. Anal. Chem., 75, 3853, 2003. and Coltro, W.K.T., et al.. Electrophoresis, 25, 3832, 2004. With permission.)... 

Ding, Y, and Garcia, C. D., Pulsed amperometric detection with poly(dunethylsiloxane)-fabricated capillary electrophoresis microchips for the determination of EPA priority pollutants. Analyst, 131, 208, 2006. 

Electrochemical Techniques, Fig. 2 Schematic diagrams of a 3-D adjustable device for the amperometric detection (AD) of microchip capillary electrophoresis (CE). (a) Glass microchip, (b) separation channel, (c) injection channel, (d) pipette tip for buffer reservoir, (e) pipette tip for reservoir not used, (/) pipette tip for sample reservoir, (g) Plexiglas holders, (h) buffer reservoir not... 

These pyrolyzed photoresist three-dimensional electrodes have applications in batteries, but they are also applicative to microchip bioanalysis systems. Fischer et al. have used pyrolyzed photoresist to make three-dimensional electrodes for dual amperometric detection for microchip electrophoresis [8]. However, it is important to note that many electrodes for microchip electrophoresis are three-dimensional electrodes and can be fabricated from a number of sources including carbon-based screen printing inks, precious metal-based screen printing inks, carbon fibers, electroplating of metallic electrodes, vapor deposition of metallic electrodes, etc. The choice of material is usually a function of... 

---