Microalgae allelochemicals

Other kinds of bloassays have been used to detect the presence of specific allelochemical effects (8), effects on N2 fIxatlon (9), the presence of volatile compounds (10) and of Inhibitory substances produced by marine microalgae (11). Putnam and Duke (12) have summarized the extraction techniques and bioassay methods used In allelopathy research. Recent developments In high performance liquid chromatography (HPLC) separation of allelochemlcals from plant extracts dictates the need for bloassays with sensitivity to low concentrations of compounds contained In small volumes of eluent. Einhellig at al. (13) described a bloassay using Lemna minor L. growing In tissue culture cluster dish wells that maximizes sensitivity and minimizes sample requirements. 

The book is divided into 3 sections. Section I.Cellular Model Systems, includes 3 chapters (Allelopathy and plant cell diagnostics, Cellular models as biosensors and microalgae for determining the effects of allelochemicals). Section II. New Methods of Microscopy has 5 chapters (Microscopic methods to study morpho-cytological events during the seed germination,... 

Aliotta, G., Della Greca, M., Monaco, P., Pinto, G., Pollio, A., and Previtera, L. 1996. Potential allelochemicals from aquatic weeds their action on microalgae. In Narwal, S. S. and Tauro, P. (Eds.), Allelopathy Field Observations and Methodology. Scientific Publishers, Jodhpur, India, 243-254. 

Most of the dimeric phenanthrenoids are C-C linked between the two units. However, phoyurmanin E (420) from whole plant of Pholidota yunnanensis ROLFE. is connected at C-1 and C-7 through an ether bond [5]. In these studies on the allelochemical interaction, between the wetland plant Juncus acutus and microalgae, six unusual dimeric 9,10-dihydro- phenanthrenoids 426-431, have been isolated and identified [190,191] as having different connection patterns. Fig. (20). 

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