High performance capillary electrophoresis micellar electrokinetic

Thormartn, W., Meier, R, Marcolli, C., and Binder, F., Analysis of barbiturates in human serum and urine by high-performance capillary electrophoresis-micellar electrokinetic capillary chromatography with on-column multiwavelength detection, /. Chromatogr., 545, 445, 1991. 

Meier, P. Thormann, W. Determination of thiopental in human serum and plasma by high-performance capillary electrophoresis-micellar electrokinetic chromatography. J. Chromatogr. 1991, 559, 505-513. 

Finally, when RPC methods are used in preparative studies with peptides, the opportunity routinely exists for subsequent analysis of the recovered fractions by a variety of analytical methods including high-speed RP-HPLC, HP-IEX, HP-HILIC, or HP-IMAC, zonal or micellar electrokinetic high-performance capillary electrophoresis (HP-CZE and MECK-CZE), capillary electrochromatography (CEC), or capillary isotachophoresis. The combination of the RPC information, drawn from the In k versus i > plots, with the data derived from on-line spectroscopic detection thus readily provides a comprehensive opportunity to assess the purity of an isolated peptide, many of the physicochemical features of the interaction, as well as a means to optimize the resolution in the RPC separation. 

Khaledi, M.G, Micellar electrokinetic chromatography, in High Performance Capillary Electrophoresis, Khaledi, M.G, Ed., Wiley-Interscience, New York, 1998, Chapter 3. 

Another format of chromatography which has been performed on electrophoresis chips is micellar electrokinetic capillary chromatography (MECC). This technique represents a very powerful extension of CE for the separation of both neutral species and ionic compounds, and has been originally developed by Terabe et al. [76]. Transfer of MECC to CE microchips has been first demonstrated by Moore et al. [77]. The experimental procedure used is identical to high speed CE, apart from the fact that a MECC buffer with 50 mM SDS and 10% methanol was used. Again three coumarin dyes were used as a model sample. Separation was achieved within a few minutes. At low applied field strengths (< 400 V/cm), the reproducibility was found to be excellent (below 1 %). 

Owing to its outstanding high-performance separation potential also capillary electrophoresis has been employed in different modes and with different detection methods for separation and determination of MC. Both capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC), which employs a surfactant above the critical micelle concentration to separate neutral and uncharged molecules based on electromigration principle, have been employed for determination of... 

ANN has recently become the focus of interest in chemistry [75], ANN, as well as PLS, also represent soft modeling without the necessity to know or to determine chemical physicochemical parameters or to establish a mathematical model. Recently, ANN has successfully been applied in capillary zone electrophoresis [76-78], ion chromatography [79], electrokinetic micellar chromatography [80], and optimization in high performance liquid chromatography (HPLC) [81], for example. We have also used ANN in equilibria, and they can also be used in extraction data analysis. 

Capillary electrophoresis with amperometric detection was also used for the determination of four electroactive preservatives (methylparaben, ethylparaben, propylparaben, and butylparaben) and, indirectly, two nonelectroactive preservatives (potassium sorbate and sodium lactate) in various types of foodstuffs [17]. Moreover, high-performance micellar electrokinetic capillary chromatography with amperometric detection (MECC-AD) has been also employed for the fast determination of melamine (2,4,6-triamino-s-triazine), which was occasionally used to increase the apparent protein content of milk products [18]. 

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