Methyltransferase gene cloning

Szumlanski C, Otterness D, Her C et al. Thiopurine methyltransferase pharmacogenetics human gene cloning and characterization of a common polymorphism. DNA Cell Biol 1996 15 17— 30. 

Yan L, Otterness DM, Kozak CA, Weinshilboum RM. Mouse nicotinamide N-methyltransferase gene molecular cloning, structural characterization, and chromosomal localization. DNA cell Biol. 1998 17(8) 639—667. 

Stover, P J., Chen, L., Suh, J Stover, D, Keyomarsi, K., and Shane, B. (1997). Molecular cloning, characterization, and regulation of the human mitochondrial serine hydroxy methyltransferase gene. I- Biol. Chem. 272, 1842-1848. 

Margison, G. P., Cooper, D. P., and Brennand, J. (1985) Cloning of the E. coli (/ -methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay. Nucleic Acids Res. 13, 1939-1952. 

Fu, J., Ding, L., Clarke, S. (1991). Purification, gene cloning, and sequence analysis of an L-isoaspar-tyl protein carboxyl methyltransferase from Escherichia coli. J. Biol. Chem. 266, 14562-14572. 

Vannini, V, Rodriguez, A., Vera, J.L., et al. (2011) Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) preUminary characterization. Biotechnol Lett... 

Koike-Takeshita, A., Koyama, T, and Ogura, K., Identification of a novel gene cluster participating in menaquinone (vitamin Kj) biosynthesis. Cloning and sequence determination of the 2-hepta-prenyl-l,4-naphthoquinone methyltransferase gene of Bacillus stearothermophilus, J. Biol Ghem., 272, 12,380, 1997. 

Muzac, I. et al.. Functional expression of an Arabidopsis cDNA clone encoding a fiavonol 3 -0-methyltransferase and characterization of the gene product. Arch. Biochem. Biophys., 375, 385, 2000. 

The general scheme of the biosynthesis of catecholamines was first postulated in 1939 (29) and finally confirmed in 1964 (Fig. 2) (30). Although not shown in Figure 2, in some cases the amino acid phenylalanine [63-91-2] can serve as a precursor it is converted in the liver to (-)-tyrosine [60-18-4] by the enzyme phenylalanine hydroxylase. Four enzymes are involved in E formation in the adrenal medulla and certain neurons in the brain tyrosine hydroxylase, dopa decarboxylase (also referred to as L-aromatic amino acid decarboxylase), dopamine-P-hydroxylase, and phenylethanolamine iV-methyltransferase. Neurons that form DA as their transmitter lack the last two of these enzymes, and sympathetic neurons and other neurons in the central nervous system that form NE as a transmitter do not contain phenylethanolamine N-methyl-transferase. The component enzymes and their properties involved in the formation of catecholamines have been purified to homogeneity and their properties examined. The human genes for tyrosine hydroxylase, dopamine- 3-oxidase and dopa decarboxylase, have been cloned (31,32). It is anticipated that further studies on the molecular structure and expression of these enzymes should yield interesting information about their regulation and function. 

Bout, S., and Vermerris, W., 2003, A candidate gene-approach to clone the sorghum Brown midrib gene encoding caffeic acid O-methyltransferase, Mol. Genet. Genomics 269 205-214. 

H Ikeda, LR Wang, T Ohta, J Inokoshi, S Omura. Cloning of the gene encoding avermectin B 5-O-methyltransferase in avermectin-producing Streptomyces avermitilis. Gene 206 175-180, 1998. 

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