Methanopyrus kandleri

Grabarse W, Vaupel M, Vorholt JA, et al. 1999. The crystal structure of methenyl-tetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri. Structure Fold Des 7 1257-68. 

Lolkema, J. S., and Boekema, E. J. (2003). The A-type ATP synthase subunit K of Methanopyrus kandleri is deduced from its sequence to form a monomeric rotor comprising 13 hairpin domains. FEBSLett. 543, 47-50. 

Property Methanobacterium thermoautotroph-icum strain AH [100] Methanosarcina barkeri strain MS [186] Methanopyrus kandleri strain MS [356]... 

Methenyl-H4MPT cyclohydrolase has been purified from M. thermoautotrophicum (AH and Marburg) [100,113], Methanopyrus kandleri[i56], and methanol-grown M. barkeri ,(>]. Properties of these purified enzymes are given in Table 8. 

Ornithine is found in addition to lysine (e.g., Methanobrevibacter smithii strain PS and Methanopyrus kandleri). In Methanobrevibacter smithii, ornithine was found to be attached to the a-carboxylic group of a glutamic acid residue (Fig. 3). 

The purification scheme is summarized in Table II. In the case of Methanopyrus kandleri reverse gyrase, the phosphocellulose step is carried out before the phenyl-Sepharose chromatography. The heparin-Sepharose step is followed with a Mono Q column and achieved with a gel filtration (Superdex 200). For Thermotoga mar-itima reverse gyrase, the purification scheme includes, in order, phosphocellulose. 

In this chapter the purification and characterization of DNA topoisomerase V from Methanopyrus kandleri cells is described. Furthermore, current applications of topoisomerase V in DNA technology are also presented, including discussion of those features of the enzyme that are of interest for DNA sequencing. 

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