Genetically engineered Saccharomyces

Friberg, A., Johanson, T., Franzen, J. etal. (2006) Efficient bioreduction of bicyclo[2.2.2]octane-2,5-dione and bicyclo[2.2.2]oct-7-ene-2,5-dione by genetically engineered Saccharomyces cerevisiae. Organic and Biomo-... 

Farhi, M., Dudareva, N., Masci, T. et al. (2006) Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae. Journal of Biotechnology, 122, 307-315. 

Production of Ethanol from Cellulosic Biomass Hydrolysates Using Genetically Engineered Saccharomyces Yeast Capable of Cofermenting Glucose and Xylose... 

The genetically engineered Saccharomyces cerevisiae 424A (LNH-ST) was used for fermentation of lignocellulosic hydrolysates to ethanol. S. cerevisiae 424A (LNH-ST) was constructed by integrating multiple copies of XD, XR, and XK into the chromosomes of S. cerevisiae ATCC 4124 accord-... 

Sedlak, M. and Ho,N.W.Y. (2004). Production of Ethanol From Cellulosic Biomass Hydrolysates Using Genetically Engineered Saccharomyces Yeast Capable of Cofermenting Glucose and Xylose. Appl. Biochem. Biotechnol., 113-116, 403-416. 

Ho, N. W. Y., Chen, Z. D., and Brainard, A. P., Genetically engineered saccharomyces yeast capable of effective cofermentation of glucose and xylose. Applied Environmental Microbiology 1998, 64 (5), 1852-59. 

Ho, N.W.Y. Chen, Z. Brainard, A.P. Sedlak, M. Genetically engineered Saccharomyces yeasts for conversion of cellulosic biomass to environmentally friendly transportation fuel ethanol. In Green Chemical Syntheses and Processes, American Chemical Society Symposium Series Anastas, P.T., Heine, L.G., Williamson, T.C., Eds. American Chemical Society Press Washington, DC, 2000 Vol. 767, 143-159 (Chapter 12). 

Over the past 30 years, a few researchers have reported the presence of xylose isomerase in a number of yeasts and fungi capable of rapid xylose metabohsm. Because of difficulties in using genetically engineered Saccharomyces, Freer et al. [38] re-examined Rhodosporidium toruloides to see if they could confirm an earlier report that this yeast produces xylose isomerase. They reasoned that the heterologous expression of an eukaryotic enzyme could fadfitate genetic engineering of xylose metabolism in S. cerevisiae. Unfortunately, they found that R. toruloides uses an oxidoreductase system fike other eukaryotes. Other approaches, however, have been more successful. 

Successful Design and Development of Genetically Engineered Saccharomyces Yeasts for Effective Cofermentation of Glucose and Xylose from Cellulosic Biomass to Fuel Ethanol... 

Successful Design and Development of Genetically Engineered Saccharomyces Yeasts... 

Fig. 4. Fermentation of glucose and xylose by genetically engineered Saccharomyces yeast strain 1400(pLNH32) [15]...

Fig. 9 A, B. Comparison of fermentation of xylose under identical conditions by A genetically engineered Saccharomyces yeast strain 1400(pLNH32) which contains the cloned and genetically modified XR, XD, and XK genes and by B 1400 (pXR-XD) which contains only the same cloned XR and XD genes, but not the cloned XK gene. These results demonstrate the importance of cloning the XK gene to enable the Saccharomyces yeasts such as 1400 (pLNH32) to ferment xylose to ethanol...

Fig. 10A, B. Comparison of cofermentation of glucose and xylose present in the medium under identical conditions by A genetically engineered Saccharomyces yeast strain 1400 (pLNH32) and by B Pichia stipitis. These results demonstrate that our genetically engineered 1400(pLNH32) can effectively coferment glucose and xylose to ethanol but not P. stipitis. Symbols square glucose circle xylose triangle ethanol...

Effective New Integration Method for the Development of Super-stable Genetically Engineered Saccharomyces Yeasts Containing Multiple Copies of the XR-XD-XK Cassette Integrated into the Yeast Chromosome... 

Fig. 11 A. Comparison of fermentation of glucose and xylose under identical conditions by A genetically engineered Saccharomyces yeast strain 259A(LNH-ST)-2 and by. ..

Genetically Engineered Saccharomyces Yeasts Able to Coferment Glucose and Xylose Present in Crude Hydrolysates of Various Cellulosic Biomass by Batch or Continuous Process... 

Fig. 12. Fermentation of glucose and xylose present in the crude hydrolysate of corn fiber by genetically engineered Saccharomyces yeast strain 1400(pLNH32). The corn fiber hydrolysate was provided by Cargill, Inc. The major sugars present in the hydrolysate are glucose, Xylose, and-arabinose. Symbols solid square glucose solid circle xylose solid triangle ethanol open circle arabinose open triangle xylitol and arabitol open square glycerol...

Ho NWY, Chen Z, Brainard AP (1998) Genetically engineered Saccharomyces yeast capable of effective cofermentation of glncose and xylose. Appl Environ Microbiol 64 1852 -1859 Takuma S, Nakashima N, Tantinmgkij M, Kinoshita S, Okada H, Seki T, Yoshida T (1991) Appl Biochem Biotechnol 28/29 327-340... 

Hepatitis B (recombinant) Vaccine is a noninfectious rDNA hepatitis B vaccine containing purified surface antigen of the virus obtained by culturing genetically engineered Saccharomyces cerevisiae cells, which carry the surface antigen gene of the hepatitis B... 

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