Mesenchymal stem cells matrices

Varghese, S., Hwang, N. S., Canver, A. C., Theprungsirikul, P., Lin, D. W., Elisseeff, J. Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells. Matrix Biology. 2008, 27, 12-21. 

S. Varghese, N.S. Hwang, A.C. Canver, P. Theprungsirikul, D.W. Lin, J. Elisseeff, Chondroitin sulfate based niches for chon-drogenic differentiation of mesenchymal stem cells, Matrix Biol. 27(1) (2008) 12-21. 

Young RG, Butler DL, Weber W, Caplan AI, Gordon SL, Fink DJ. Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair. J Orthop Res 1998 16 406 13. 

Fig. 15.1 (a) A simplified scheme of an embryonic renal progenitor unit consisting of metanephric mesenchymal stem cells and ureteric buds, which cross-talk via growth factors (GFs) and their receptors, molecules of the extracellular matrix (ECM) and specific integrins, proto-oncogenes and specific ligands and give rise to the differentiated cell types of the adult kidney (see text). 

Prante C, Bieback K, Fiinke C, Schon S, Kern S, Kuhn J, Gastens M, Kleesiek K, Getting C. The formation of extracellular matrix during chondro-genic differentiation of mesenchymal stem cells correlates with increased levels of xylosyltransferase I. Stem Cells 2006 24 2252-2261. 

Wollenweber, M. Domaschke, H. Hanke, T. Boxbetger, S. Schmack, G. GUesche, K. Schamweber, D. Worch, H. Mimicked bioartificial matrix containing chondroitin sulphate on a textile scaffold of poly(3-hydroxybutyrate) alters the differentiation of adult human mesenchymal stem cells. Tissue Eng. 2006 February, 12(2), 345-359. 

The mechanical properties of the capsule must be carefully considered. Not only does the capsule need sufficient strength to resist mechanical failure and rupture but the elasticity of cell substrates have been shown to influence cell behavior. The mechanism by which substrate elasticity influences differentiation of multipotent cells is not clear. A number of recent studies have analyzed this in both two- and three-dimensional (3D) culture systems, - Integrin engagement is thought to be required to transmit the elasticity of the capsule material to the cells inside. Matrix elasticity has been shown to influence the lineage commitment of naive, mesenchymal stem cells. Another important consideration is how the mechanical properties of a capsule impact the transmission of external mechanical forces to the cells. Many cells respond to mechanical loads and this can be necessary for maintenance of phenotype or to guide differentiation. ... 

Compte M, Cuesta A M, Sanchez-Martin D, Alonso-Camino V, Vicario J L, Sanz L and Alvarez-Vallina L (2009), Tumor immunotherapy using gene-modified human mesenchymal stem cells loaded into synthetic extracellular matrix scaffolds . Stem Cells, 27, 753-60. 

Liu Y, Shu X Z and Prestwich G D (2006), Osteochondral defect repair with autologous bone marrow-derived mesenchymal stem cells in an injectable, in situ, cross-Unked synthetic extracellular matrix . Tissue Eng, 12, 3405-16. 

Silk-elastin protein polymers can also be used to encapsulate mammalian cells and direct cellular behavior. Haider et al. encapsulated human mesenchymal stem cells by suspending cells in a solution of the protein polymer and incubating the solution at 37 °C to induce hydrogel formation. The scaffolds induced chondrogenesis of the encapsulated cells when cultured for up to 4 weeks in chondrogenic media. Silk-elastin protein polymers are also amenable to injection and, thus, could be directly delivered to specific areas of the body to form a dmg- or cell-loaded matrix that will degrade slowly over... 

Anderson, J.M., Kushwaha, M., Tambralli, A., Beilis, S.L., Camata, R.P., Jun, H.W. Osteogenic differentiation of human mesenchymal stem cells directed by extracellular matrix-mimicking ligands in a biomimetic self-assembled peptide amphiphile nanomatrix. Biomacromolecules 10, 2935-2944 (2009)... 

Kim, J., Kim, H.N., Lim, K.T., Kim, Y., Seonwoo, H., et al. Designing nanotopo-graphical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells. Scientific Reports 3, 3552 (2013)... 

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