MCF-7 cell lines

The cytotoxicity of other metal complexes was also investigated. Neundorf, Schatzschneider, and coworkers found that the cymantrene derivative of the branched cell-penetrating peptide hCT(18-32)-k7 mentioned in Sect. 2.2.1 shows high cytotoxicity (IC50 = 36 pM) against the MCF-7 cell line, while the cymantrene complex as well as the peptide alone is nontoxic [47]. Likewise, the attached... 

It was shown by previous investigations that use of Platidiam (Ptd) solutions with a concentration of C = 1.5 pg/mL corresponds to an IC50 dose for the MCF-7 cell line. Therefore, makeup Ptd solution (water for injections) of mentioned concentration (A dose) and consecutively halved concentrations (A and A doses, respectively) was used in in vitro experiments. We also studied the influence of the... 

Acylation of free hydroxyl at C-7 in paclitaxel usually led to the reduction and even loss of cytotoxicity of the derivatives, when steric hindrance of the acyl groups increases. Bhat et al. have used a parallel solution phase synthetic method to construct a 26-membered library of C-7 esters, and concluded that modification at C-7 were detrimental to cytotoxicity against the MCF-7 cell line. Only a few exceptions, including 10-deacetyl-lO-propionyl-7-chloroacetyl... 

Rat pancreatic carcinoma AR42J, human colon carcinoma HT-29 and human breast carcinoma MCF-7 cell lines were procured from the National Centre for Cell Science (Pune, India). All cell cultures for in vitro studies were maintained in the in-house biosafety facility at the BARC. 

A binding of 13.2 0.8% ( = 3) was observed with 5 x 10 MCF-7 cells for 1 gg of the Lu-p-NCS-benzyl-DOTA-estradiol complex. With SepPak purification, the cell uptake improved to 17.1 1.6% (n = 3). No further increase in cell uptake was observed with HPLC purification. It was observed that the tracer uptake in the cells decreased to 8.3 2.0% (n = 3) with the addition of 100 gg of cold estradiol. Similar results were observed when these experiments were carried out on 24 well plates where cells were plated 1 d prior to the experiments. The decrease in cell uptake with the addition of estradiol indicated the specificity of the radiolabelled conjugate for the MCF-7 cell lines. Blank experiments were carried out with Lu labelled BFCA under similar experimental conditions. No retention of the activity in the cell pellet was observed, ruling out the possibility of carrier mediated uptake. 

All. Asoh, K., Watanabe, Y., Mizoguchi, H., Mawatari, M Ono, M., Kohno, K., and Kuwano, M., Induction of manganese superoxide dismutase by tumor necrosis factor in human breast cancer MCF-7 cell line and its TNF-resistant variant. Biochem. Biophys. Res. Common. 162,794-801 (1989). 

Human epithelial antigen VU-1 D9 34- and 49-kD glycoproteins on the surface and in cytoplasm of most epithelial cells (except squamous epithelium, hepatocytes, and parietal cells) MCF-7 cell line Ventana NA HIER... 

Jiang L, Chen RS, Li JC. siRNA-cyclin D1 inhibit cell proliferation in breast cancer MCF-7 cell line. Fen Zi Xi Bao Sheng Wu Xue Bao 2006 39 118-122. 

PEAA-co-XMA (X = butyl or hexyl). The cationic block, based on trimethylammonium ethyl methacrylate, was also modified with hydrophobic groups. These polymers showed the ability to silence GAPDH expression upon the deUvery of anti-GAPDH siRNA in the MCF-7 cell line. 

The MCF-7 cell line is a human breast cancer cell line with differentiation potential [8, 9] that provides a system to study in vitro the interaction of the process of ADP-ribosylation with reagents that may modify the adenylate cyclase system and a system to study the effect of hormonal manipulation of cellular growth and differentiation on these same processes. 

Sanoj et al. have investigated the in vitro anticancer potential of curcumin loaded onto biodegradable thermosensitive nanocarriers (Fig. 5) on oral cancer (KB), prostate cancer (PC3), and breast cancer (MCF-7) cell lines. They formulated... 

C. grevei Bill, and Courchet. (Rutaceae) EO exhibited cytotoxic effects against MCF-7 cell line (human breast cancer cells) with IC50 (inhibitory concentration 50%) values of 21.5 + 2 mg/L. The cytotoxic activity of the EO may be attributed to synergistic effects of all terpenes (Afoulous et ah, 2013). Aydin et al. (2013b) tested the cytotoxic effect of terpinolene, the main compound of many aromatic plants. It showed cytotoxic effects at concentrations of 100, 200, and 400 mg/L in primary rat neurons. In N2a (neuroblastoma) cells terpinolene showed cytotoxic effects already at a concentration of 50 mg/L. The antiproliferative effects of carvone were also tested. High doses of carvone (100, 200, and 400 mg/L) showed high cytotoxic effects on N2a cell lines (Aydin et al.. 

Molybdocene dichloride (CP2M0CI2) is another cytotoxic metal complex that has been evalnated for its in vitro biological activity following Q[7] encapsnlation. Cp2MoCl2 Q[7] was fonnd to have improved activity when compared to free CP2M0CI2 with the 2008 cell line and the MCF-7 cell line. It is not known whether the improvement is related to solnbility or membrane permeability. ... 

Farabegoli F, Papi A, Bartolini G, Ostan R, Orlandi M (2010) (-)-Epigallocatechin-3-gallate downregulates Pg-P and BCRP in a tamoxifen resistant MCF-7 cell line. Phytomedicine 17 356-362... 

MCF-7 cell line in six-well plates is " C-genistein Cg column (30 cm X 4.6 mm ID), Genistein inhibits breast Peterson o 3... 

Nunez MC, Diaz-Gavilan M, Conejo-Garcia A, Cruz-Lopez O, Gtillo MA, Espinosa A, Campos JM (2008) Design, synthesis and anticancer activity against the MCF-7 cell line of benzo-fused 1,4-dihetero seven- and six-membered tethered pyrimidines and purines. Curr Med Chem 15 2614-2631... 

We investigated the reactions on the MG-63 cell line and MCF-7 cell line in mixture with various amount of OA solution. DMEM-HG was used for cell culture as the basic media. The concentration of mixture was limited from 0% to 30%. DMEM-HG volume is fixed for minimizing its effect on cells. Total culture term was 14 days and MTT assay was performed on day 1, 3, 7, 10 and 14 throughout the experiment. For cell viability test, ELISA reader was used to measure the optical density (OD) at 595nm wavelength filter. 

The development of the MCF-7 cell line, as well as others, ushered in a busding period of research to unlock the mechanisms of estrogen-based carcinogenicity. In 1975, scientists demonstrated that the antiestrogen tamoxifen inhibited the growth of MCF-7 cells. This finding had profound implications, as tamoxifen, which competes with estradiol to bind to the estrogen receptor, subsequently went on to become a standard component of therapy for virtually... 

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