Matrix-assisted laser desorption imaging

Sugiura Y, Shimma S, Setou M. Two-step matrix application technique to improve ionization efficiency for matrix-assisted laser desorption/ionization in imaging mass spectrometry. Anal. Chem. 2006 78 8227-8235. 

In a separate study, a protocol for Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) has been proposed.18 This IMS technique provides a new approach to visualize spatial distribution of thousands of molecular species, including peptides, proteins, and their metabolites in two- or three-dimensional levels. This approach may also provide a straightforward method of determining the tissue distribution of multiple peptides or proteins in a quantitative manner.18 Chu et al.19 reported a nondestructive molecular extraction method to obtain proteins from a single FFPE or frozen tissue section, without destroying the tissue morphology, such... 

The second advantage is the possibility of performing chemical imaging analyses. Even if other techniques, such as matrix-assisted laser desorption/ionization, also allow images to be acquired, ToF-SIMS is, for now, the mass spectrometric technique with the best spatial resolution performance. 

A modified version of 2DE and gel image analysis, with silver staining, autoradiography, and protein identification and measurement of peptide mass, uses matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as a rapid and sensitive technique for identifying peptides. MALDI-TOF-MS applies well to protein detection in biological fluids.56 A second advantage of this technique is... 

Site has good software subdivision devoted to matrix-assisted laser desorption/ ionization mass spectrometric imaging (MALDI-MSI, also termed imaging MS or MS imaging). 

Puretzky, A.A. Geohegan, D.B. Hurst, G.B. Buchanan, M.V. Luk yanchuk, B.S. Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption A Plume Sharpening Effect. Phys. Rev. Lett. 1999,83,444-447. 

Bunch, J., Clench, M. R., and Richards, D. S. (2004). Determination of pharmaceutical compounds in skin by imaging matrix-assisted laser desorption/ionisation mass spectrometry. Rapid Commun. Mass Spectrom. 18 3051-3060. 

Cheng, Z., Winant, R. C., and Gambhir, S. S. (2005). A new strategy to screen molecular imaging probe uptake in cell culture without radiolabeling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Nucl. Med. 46 878-886. 

Crossman, L., McHugh, N. A., Hsieh, Y., Korfmacher, W. A., and Chen, J. (2006). Investigation of the profiling depth in matrix-assisted laser desorption/ionization imaging mass spectrometry. Rapid. Commun. Mass. Spectrom. 20 284-290. 

Hsieh, Y., Casale, R., Fukuda, E., Chen, J., Knemeyer, I., Wingate, J., Morrison, R., and Korfmacher, W. (2006a). Matrix-assisted laser desorption/ionization imaging mass spectrometry for direct measurement of clozapine in rat brain tissue. Rapid Commun. Mass Spectrom. 20 965-972. 

Prideaux, B., Clench, M. R., Carolan, V. A., Morton, J., and Rajan-Sithamparanadarajah, B. (2005). Imaging matrix assisted laser desorption ionisation—mass spectrometry for the investigation of dermal absorption of chlorpyrifos. In Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, San Antonio, TX. 

Todd, P. J., Schaaff, T. G., Chaurand, P., and Caprioli, R. M. (2001). Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization. J. Mass Spectrom. 36 355-369. 

MALDI MCM-41 MCR MD ME MEM MI MPM MRI MS MVA Matrix-assisted Laser Desorption/Ionization Mobile Crystalline Material-41 Multivariate Curve Resolution Molecular Dynamics Matrix-enhanced Magnetic Force Micrscopy Multivariate Image Multiphoton Microscopy Magnetic Resonance Imaging Mass Spectroscopy Multivariate Analysis... 

Stoeckli, M. Farmer, T. B. Caprioli, R. M. 1999. Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument. J. Am. Soc. Mass Spectrom., 10, 67-71. 

Use of Matrix Assisted Laser Desorption/ Ionization Imaging Mass Spectrometry (MALDI-IMS) in the Development of Novel Small Molecule Drugs... 

Matrix assisted laser desorption/ionization imaging mass spectrometry... 

Use of Matrix Assisted Laser Desorption/Ionization Imaging Mass Spectrometry... 

Burrell M, Earnshaw C, Clench M (2007) Imaging matrix assisted laser desorption ionization mass spectrometry a technique to map plant metabolites within tissue at high spatial resolution. J Exp Bot 58(4) 757-763. doi 10.1093/jxb/erll39... 

Trim P, Henson C, Avery J, McEwen A, Snel M, Claude E, Marshall P, West A, Princivalle A, Clench M (2008) Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections. Anal Chem 80 8628-8634. doi 10.1021/ac8015467... 

Drexler D, Garrett T, Cantone J, Diters R, Mitroka J, Conaway M, Adams S, Yost R, Sanders M (2007) Utility of imaging mass spectrometry (IMS) by matrix-assisted laser desorption ionization (MALDI) on an ion trap mass spectrometer in the analysis of drugs and metabolites in biological tissue. J Pharmacol Toxicol Methods 55 279-288. doi 10.1016/j. vascn.2006.11.004... 

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