Matrigel matrix

We have used the Matrigel matrix reconstituted with BMPs/OPs for a novel local treatment of systemic bone loss in our cohort of OVX primates Papio ursinus [62]. The bone mineral density of the lumbar vertebrae of the OVX primates was significantly affected by estrogen depletion [62]. Histomorphometric data on iliac crest biopsies showed that bone was permanently lost 36 months after ovariectomy. Injections of 0.5 mg of naturally derived BMPs/OPs into the lumbar vertebrae 3 and 4 using an X-ray image intensifier for guidance was successfully performed for the first time in primates [62] (Fig. 7). This novel method may prove to be valuable for the treatment of systemic bone loss by localized injections of osteogenic proteins of the superfamily in clinical contexts. 

Corning Matrigel Matrix (standard formulation, phenol red free) Chemokine solution For live-cell imaging... 

Figure 2 Filling of chamber (A and B) first half of the chamber is filled with Matrigel matrix containing cells and chemokine, (C) after polymerization of the gel, the second half of the chamber is filled with Matrigel containing chemokine only, and (D) the chamber is sealed with paraffin.

Rotating culture vessels such as simulated microgravity systems are primarily used to study 3-D tumor growth and differentiation. However, mixed cell populations combined with matrix proteins can be used to generate a complex microenvironment in which cell-cell interactions and invasion can be measured (95). A similar system has also been described for the coculture of endothelial cells, myofibroblasts, and tumor cell clusters embedded in Matrigel . Differential labeling of the cell populations enables their invasion and the effects of inhibitors to be measured (96). 

We and others have based invasion assays on preformed tumor spheroids generated either in hanging drops (88) or other nonadherent systems (61). These are then transferred to matrix monolayers or embedded in Matrigel and can be analyzed qualitatively or quantitatively, usually by microscopy and image analysis (see also Chapter 16). A96- or 1,536-well spheroid-based... 

Matrigel Basement Membrane Matrix (BD Biosciences, San Jose, CA). 

Vukicevic, S. et al. 1992. Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular activity related to extracellular matrix components. Exp. Cell Res. 202, 1-8. 

The Boyden-chamber chemoinvasion sissay, developed by Albini et al. (1987) using Matrigel , is an alternative simplified model system for measuring cell capacity to attach to extracellular matrix, degrade it, and migrate towards a chemoattractant. Matrigel is a... 

These invasion steps are most easily studied on amnion complete with epithelium, or in bioengineered cell monolayers. These can be easily produced by seeding vascular endothelial cells (EC) onto reconstituted extracellular matrix (Matrigel ), or corneal EC onto bovine lens capsule mounted in a combi-ring dish. 

It is interesting to note that from co-injection experiments with Matrigel , it has emerged that the cooperative effects of host fibroblasts is exerted not only through direct secretion of growth factors, but also by release of proteases that can liberate growth factors adsorbed to the extracellular matrix, making them available to the cancer cells (Noel et al., 1998). 

Hepatocytes are attachment-dependent cells [3, 25]. Without adequate extracellular matrix they lose their liver-specific functions. Therefore, it has been studied intensively which types of matrix, hydrogels, and scaffolds support differentiated hepatocyte functions and which are the key properties. One of the first commercially available hydrogels was Matrigel, an extract from mouse sarcoma cells [26]. Later Extragel, another collagen-based hydrogel [27] Alimatrix, a porous 3D scaffold [28] and PuraMatrix... 

Matrigel is the trade name (BD Biosciences) for a solubilised basement membrane preparation extracted from the Engelbreth-Hohn-Swarm mouse sarcoma, a tumor rich in extracellular matrix proteins. This mixture resembles the complex extracellular environment found in many tissues and is used as a substrate for cell culture. 

MALDI-TOF (matrix assisted laser desorption ionization time of flight) 51, 139 manufacture 244 manufacturers (CMOs) 129 market authorization application (MAA) 123 marketing authorization 205 mass spectrometry (MS) 104 mass spectroscopic analysis 175 master 67 master cell bank 139 matrigel 282 measles 63 mechanical 36 media components 104... 

---