Mass spectrometry for protein characterization

Zhang, S. Van Pelt, C. K. Chip-based nanoelectrospray mass spectrometry for protein characterization. Exp. Rev. Proteom. 2004, 1, 449-468. 

Mann, M. Wilin, M. (1995) Electrospray mass spectrometry for protein characterization. Trends Biochem. Sci. 20, 219-224. 

L.Y Yang, C.S. Lee, S.A. Hofstadler, L. Pasa-Tolic and R.D. Smith, Capillary isoelectric focusing-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for protein characterization. Analytical Chemistry, 70, 3235-3241 (1998). 

Zabet-Moghaddam, M. et al.. Matrix-assisted laser desorption/ionization mass spectrometry for the characterization of ionic liquids and the analysis of amino acids, peptides and proteins in ionic liquids. /. Mass Spec., 39,1494, 2004. 

Non-volatile organic compounds can be characterized by c.g.c. if pyrolyzed directly into the injector chamber of a g.c. [56, 57 ]. It has been shown that size exlusion chromatography and gel permeation chromatography are adequate techniques for fractionation of non-volatile components from water samples. The fractions are then to pyrolysis-g.c.— mass spectrometry for the characterization of humic acid and fulvic acids, sugars, and proteins [57 ]. ... 

The first two protocols presented in this work focus on high-throughput gel-based techniques. Chapter 1 describes 2-D DICE, a method used to examine the entire proteome within discreet pi fractions, whereas Chapter 2 details several complementary techniques for the specific analysis of glycoproteins found in tissue samples. The next chapters. Chapters 3 and 4, focus on SELDI-MS and MALDI-TOF, two techniques that make use of mass spectrometry for the characterization and identification of proteins. These techniques may be used alone or in combination with other proteomic methods, such gel-based assays, where mass spectrometry is useful for additional characterization and identification of proteins. 

See Volume 5, Chapter 4 Chemical Derivatization and Multistage Tandem Mass Spectrometry for Protein Structural Characterization by Jennifer M. Froelich, Yali Lu, and Gavin E. Reid. 

A Chemical Derivatization and Multistage Tandem Mass Spectrometry for Protein Structural Characterization... 

Leymarie, N. Berg, E.A. McComb, M.E. O Conner, P.B. Grogan, J. Op-penheim, F.G. Costello, C.E. Tandem Mass Spectrometry for Stractural Characterization of Proline-Rich Proteins Application to Salivary PRP-3. Anal. Chem. 2002, 74,4124-4132. 

Opiteck, G.J., Jorgenson, J.W., MacNair, J.E., Moseley, M.A., 3rd (1997). Two-dimensional SEC/RPLC coupled to mass spectrometry for the analysis of peptides. Rapid separation and characterization of protein and peptide mixtures using 1.5 microns diameter non-porous silica in packed capillary liquid chromatography/mass spectrometry. Anal. Chem. 69, 2283-2291. 

Fang X, Yang L, Wang W, et al. Comparison of electrokinetics-based multidimensional separations coupled with electrospray ionization-tandem mass spectrometry for characterization of human salivary proteins. Anal. Chem. 2007 79 5785-5792. 

Thomas, J.J., Shen, Z., Crowell, J.E., Finn, M.G., Siuzdak, G. Desorption/ ionization on silicon (DIOS) a diverse mass spectrometry platform for protein characterization. Proc. Nad. Acad. Sci. USA 2001, 98, 4932-4937. 

To facilitate our understanding of gene function, large-scale analyses of proteins are also necessary. Proteomics is currently divided into three main areas (1) mass spectrometry for characterization and identification of proteins (2) differential display proteomics for comparison of protein levels and (3) studies of protein-protein interaction by using the yeast two-hybrid system or phage display technology. 

Recent developments in instrument design have led to lower limits of detection, while new ion activation techniques and improved understanding of gas-phase ion chemistry have enhanced the capabilities of tandem mass spectrometry for peptide and protein structure elucidation. Future developments must address the understanding of protein-protein interactions and the characterization of the dynamic proteome (Chalmers and Gaskell 2000). 

The analytical strategies for protein characterization rely heavily on high-performance liquid chromatography (HPLC) and/or electrophoretic separation of proteins/peptides, followed by other detection methods [e.g., mass spectrometry (MS)]. 

Davis, M.T. Stahl, D.C. Swiderek, K.M. Lee, T.D. Capillary Liquid Chro-matography/Mass Spectrometry for Peptide and Protein Characterization, Methods A Companion to Methods in Enzymology 6,304-314 (1994). 

Mass Spectrometry Provides Powerful Tools for Protein Characterization and Identification... 

Gennaro, L.A., Salas-Solano, O. and Ma, S. (2006) Capillary electrophoresis-mass spectrometry as a characterization tool for therapeutic proteins. Anal Biochem, 355 (2), 249-258. 

Bou-Asaf, G.M., Marshall, A.G. (2014) Biophysical mass spectrometry for biopheumaceutical process development Focus on hydrogen/deuterium exchange, in Biophysical Characterization of Proteins in Developing Biopharmaceuticals (eds D. J. Houde and S. A. Berkowitz), Elsevier, Amsterdam, pp. 307-339. 

---