Nanoelectrospray chip-based

Zhang, S. Van Pelt, C. K. Chip-based nanoelectrospray mass spectrometry for protein characterization. Exp. Rev. Proteom. 2004, 1, 449-468. 

Zhao, W., Zhang, H., Zhu, M., Warrack, B., Ma, L., Humphreys, W. G., and Sanders, M. (2006). An integrated method for quantification and identification of radiolabeled metabolites Application of chip-based nanoelectrospray and mass defect filter techniques. In Proceedings of the 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA. 

Balimane PV, Pace E, Chong S, Zhu M, Jemal M, Pelt CK. A novel high-throughput automated chip-based nanoelectrospray tandem mass spectrometric method for PAMPA sample analysis. J Pharm Biomed Anal 2005, 21 Jun [Epub]. 

Hop, C.E., Chen, Y., and Yu, L.J., Uniformity of ionization response of structurally diverse analytes using a chip-based nanoelectrospray ionization source, Rapid Commun. Mass Spectrom., 19(21), 3139, 2005. 

Hop et al. [56,59] demonstrated that ESI response factors could be normalized using nanoelectrospray. In this report, 25 compounds from 6 structurally distinct classes were tested using silicon chip-based nanoelectrospray devices (NanoMate) with <300 nL/min flow rate. The MS responses between these 25 compounds were relatively consistent (within 2.2-fold). This can be compared to standard LC-MS data which would have a 21-fold difference in response therefore, it was a big improvement. 

Amon, S., TreUe, M.B., lensen, O.N., J0rgensen, T.l. (2012) Spatially resolved protein hydrogen exchange measured by subzero-cooled chip-based nanoelectrospray ionization tandem mass spectrometry. Anal Chem, 84 (10), 4467-A473. 

Another technique that shows potential for use in the EDM assay arena is the nanoelectrospray technology. Nanoelectrospray has been available for many years but has recently become available in a convenient chip-based system that can be used for both quantitative and qualitative applications (Dethy et al., 2003 Ackermann and Dethy, 2005 Wickremsinhe et al., 2005, 2006). The main advantage of this technique is that it is fairly easy to implement (it can be interfaced directly to many triple quadrupole MS systems) and it provides a fast assay time (about 20—30 s/sample). Van Pelt et al. (2003) described the use of the nanoelectrospray system for the analysis of Caco-2 samples. More reeently, Balimane et al. (2005) demonstrated that a chip-based nanoelectrospray system could be used to assay PAMPA samples in a high-throughput maimer. In this report, the samples for 10 test compounds were assayed by HPLC UV or nanoelectrospray and the permeability results from the two assays were found to be similar for this test set. 

Himmelsbach, M., Varesio, E., and Hopfgartner, G. 2014. Liquid extraction surface analysis (LESA) of hydrophobic TLC plates coupled to chip based nanoelectrospray high resolution mass spectrometry, Chimia, 68 150-154. 

Zamfir, A., Vukehc, Z., Bindila, L., Peter-Katalinic, J., Almeida, R., Sterling, A. and Ahen, M., FuUy-automated chip-based nanoelectrospray tandem mass spectrometry of ganghosides from human cerebeUum. J. Am. Soc. Mass Spectrom., 15, 1649-1657 (2004). 

Nano-ESI is a variant of ESI that uses smaller spray capillaries (and hence smaller flows, often below 1 juL/min, thus the term nano ), and it produces smaller initial droplets. This is beneficial for the investigation of noncovalent complexes. In particular for quantitative studies, the situation where less than one molecule is contained in a droplet, and thus the point where the spray and desolvation processes cannot distort an equilibrium any more, will be reached much faster for nano-ESI than for ESI. A variant of nano-ESI is a fully automated, chip-based nanoelectrospray system that has recently become available commercially. Only... 

VuKELic, Zarei M. Peter-Katalinic, J. Zamfir, A. D. Analysis of human hippocampus gangliosides by fully-automated chip-based nanoelectrospray tandem mass spectrometry. J. Chromatogr. A 2006, 1130, 238-245. 

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