Tandem mass spectrometry multistage

Principles and Characteristics Analytical multistage mass spectrometry (MSn) relies on the ability to activate and dissociate ions generated in the ion source in order to identify or obtain structural information about an unknown compound and to analyse mixtures by exploiting two or more mass-separating steps. A basic instrument for the currently most used form, tandem mass spectrometry (MS/MS), consists of a combination of two mass analysers with a reaction region between them. While a variety of instrument set-ups can be used in MS/MS, there is a single basic concept involved the measurement of the m/z of ions before and after a reaction in the mass spectrometer the reaction involves a change in mass and can be represented as ... 

Leenheer, J. A., Rostad, C. E., Gates, P. M., Furlong, E. T., and Ferrer, I. (2001). Molecular resolution and fragmentation of fulvic acid by electrospray ionization/multistage tandem mass spectrometry. Anal. Chem. 73,1461-1471. 

Scherperel, G., Yan, H.G., Wang, Y. and Reid, G.E. (2006) Top-down characterization of site-directed mutagenesis products of Staphylococcus aureus dihydroneopterin aldolase by multistage tandem mass spectrometry in a linear quadrupole ion trap. Analyst, 131, 291-302. 

See Volume 5, Chapter 4 Chemical Derivatization and Multistage Tandem Mass Spectrometry for Protein Structural Characterization by Jennifer M. Froelich, Yali Lu, and Gavin E. Reid. 

A Chemical Derivatization and Multistage Tandem Mass Spectrometry for Protein Structural Characterization... 

Tandem mass spectrometry is not restricted to two stages of mass analysis (i.e., MS/MS or MS ) it is also possible to perform multistage MS (i.e., higher-order MS) experiments, abbreviated as MS". These experiments can determine the genealogical relation between a precnrsor and its ionic prodncts. For example, MS indicates three stages of tandem mass spectrometry, which involves mass selection of one of the prodncts, say either mi+,m2" ", or m3+, formed from... 

Tandem mass spectrometry is a multistage mass spectrometry technique that can precisely ascertain the progeny of ions. MS/MS involves mass selection of a precursor ion, activation and fragmentation of the mass-selected precursor, and mass analysis of the charged products. This technique can be applied to... 

Kennedy, J.H. and Wiseman, J.M. 2010. Direct analysis of Salvia divinorum leaves for salvinorin A by thin layer chromatography and desorption electrospray ionization multistage tandem mass spectrometry. Rapid Commun. Mass Spectrom., 24 1305-1311. 

Zehl M, Pittenauer E, Jirovetz L, Bandhari P, Singh B, Kaul VK et al (2007) Multistage and tandem mass spectrometry of glycosylated triterpenoid saponins isolated from bacopa monnieri comparison of the information content provided by different techniques. Anal Chem 79 8214-8221... 

Yan, J., Liu, Z., Yan, C., et al (2006) Analysis of strychnos alkaloids using electrospray ionization Fourier transform ion cyclotron resonance multistage tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 20,1335-1344. 

ESI tandem MS stands for electro spray ionization mass spectrometry performed in multistage. This technique is conducted based on the production of multiply charged ions from proteins and peptides. In this technique, ionization procedure is carried out within the instrument. Three types of mass analyzers are used individually or in combination. 

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