Mannose binding

Matsushita M, Hijikata M, Ohta Y, Iwata K, Matsumoto M, Nakao K et al. Hepatitis C virus infection and mutations of mannose-binding lectin gene MBL. Arch Virol 1998 143[4] 645—651. 

J. Lehmann and U. P. Weitzel, Synthesis and application of a-D-mannosyl clusters as photoaffinity ligands for mannose-binding proteins Concanavalin A as a model receptor, Carbohydr. Res., 294 (1996) 65-94. 

I. P. Fraser, H. Koziel, and R. A. B. Ezekowitz, The serum mannose-binding protein and the macrophage mannose receptor are pattern recognition molecules that link innate and adaptive immunity, Semin. Immunol., 10 (1998) 363-372. 

Fig. 3 SPR sensorgrams. Upper Binding curves of 20 pg/mL of BclA to immobilized PAA-mannose in the presence of (A) 1.95 pM-0.25 mM a-benzyl-mannoside (the best ligand from tested monosaccharides) and (B) 0.95 mM-25 mM D-galactose (non-binder). Bottom SPR sensorgrams for D-mannose binding to immobilized BclA. (C) Equilibrium steady state curves for D-mannose varying from 1 to 500 pM. (D) The corresponding binding curve derived from steady-state equilibrium values.

Figure 14.6 Representative mass spectrum showing ladder of a bisglycosylated glycopeptide obtained from screening of a glycopeptide library for the mannose binding protein. The nonanoic acid (Non) and tridecanoic acid (Tri) encode for Ser(Man) (Sm) and Asn(Man) (Nm), respectively.

As antiadhesin and inhibitors unless stated otherwise. 6 MBP Mannose binding protein. 

Affinity-based CEC separations for the analysis of mannose-binding proteins... 

Bedair, M., and El Rassl, Z. (2004). Affinity chromatography with monolithic capillary columns I. Polymethacrylate monoliths with Immobilized mannan for the separation of mannose-binding proteins by capillary electrochromatography and nano-scale liquid chromatography. /. Chromatogr. A 1044, 177-186. 

Lectin pathway, stimulated by binding to a lectin. Lectins belong to a family of proteins called collectins, which are present in blood and bind to bacteria. One lectin, known as the mannose binding lectin (MBL), binds to a sequence of mannose sugars that are part of the carbohydrate on the cell surface of some bacteria. It is the lectin-bacteria complex that activates one of the complement proteins. The components of the pathway are prefixed with a C and a number. 

Polythiophenes functionalized with monosaccharides have been evaluated for their ability to detect the influenza virus and E. coli (Baek et al. 2000). Copolymers of thiophene acetic acid 10 and carbohydrate-modified thiophenes 11 have been prepared via iron(III) chloride mediated polymerization. Addition of influenza virus to a sialic acid containing copolymer resulted in a blue shift of the polymer absorption maximum, resulting in an orange to red chromatic transition. Mannose-containing polythiophenes underwent color changes upon the addition of the lectin ConA or E. coli cells that contain cell surface mannose-binding receptors. A similar biotinylated pol5hhiophene afforded a streptavidin responsive material (Paid and Leclerc 1996). 

A number of carbohydrate recognition domains (CRDs) of animal lectins are known to exist as clusters. ASGP-Rs exist as bundle of hexamers, mannose 6-phosphate receptor and galectin-3 are known as dimers, while mannose binding proteins and receptors form multiple CRDs [31]. 

Systematic investigations of mannose-binding proteins with clustered mannosides other than natural multiantennary oligosaccharides have been rather scarce until recently. However, using 6 -0-phosphorylated mannose disaccharide scaffolded on peptide templates using combinatorial glycopeptide libraries. 

To further investigate multivalent mannose-binding interactions, we have recently begun the systemic syntheses of mannoside clusters of various shapes. 

Turner, M. W. (2003). The role of mannose binding lectin in health and disease. Mol. Immunol. 40, 423-429. 

Wallis, R., Shaw, J. M., Uitdehaag, J., Chen, C. B., Torgersen, D., and Drickamer, K. (2004). Location of the serine protease-binding sites in the collagen-like domain of mannose-binding protein. J. Biol. Chem. 279, 14065—14073. 

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