Peptide template

Pseudopeptidic Hydroxamic Acids and N-formyl-N-hydroxylamines Non-peptidic Templates... 

Scheme 3. Grb2-targeted j3 turn peptide template used for evaluation of pTyr mimics 11,13, and 17...

Rather than using the protein molecule as a whole, the imprinting of selected protein epitopes may present a more practical approach. Imprints of such patches may then act as receptors for these parts of the protein. It could be shown that an MIP imprinted with a tetrapeptide was able to recognize not only the template but also a protein bearing the same 3-amino acid terminus as the peptide template [129]. If this approach proves to be successful in other cases as well, MI-based recognition will no longer be limited to small molecules. The result will be even more antibody like biomimetic polymers. 

Systematic investigations of mannose-binding proteins with clustered mannosides other than natural multiantennary oligosaccharides have been rather scarce until recently. However, using 6 -0-phosphorylated mannose disaccharide scaffolded on peptide templates using combinatorial glycopeptide libraries. 

Synthesis of Mannose 6-Phosphate Containing Disaccharide Threonine Building Blocks and Their Use in Peptide Template Synthesis... 

The original system is based on peptides that contain heptad repeats, where the first and fourth positions of the repeat are hydrophobic amino acids. Such sequences form a-helices, which assemble into coiled-coil structures, as represented in Figure 7.10. The principle is then the same as that used for von Kiedrowski s self-replicating nucleotides (von Kiedrowski 1986), in the sense that a full-length peptide template (having in this case 32-35 residues) directs the condensation of the two half-length peptide substrates. 

Levy, M. and Ellington, A. D. (2003). Peptide-template nucleic acid ligation, / Mol Evol, 56, 607-15. 

M. Halkes, . H. Gotfredsen, M. Grotli, L. P. Miranda, J. O. Duus and M. Meldal, Solid-phase glycosylation of peptide templates and on-bead MAS-NMR analysis Perspectives for glycopeptide libraries, Chemistry-A European Journal, 2001, 7, 3584-3591. 

Along the same lines, an artificial ion channel was prepared by Montal and coworkers [33] using the TASP approach. In their work, a four a-helix bundle structure 67 was synthesized on a peptide template. The ion transport ability was well characterized and 67 turned out to have several similarities with the natural acetylcholine receptor channel they were mimicking. 

Synthesis of a Scaffolded Positional-Scanning Peptide Library Bnilt on a Bicyclic Peptide Template )... 

Schleyer, A, Meldal, M, Manat, R, Paulsen, H, Bock, K, Direct sohd-phase glycosylations of peptide templates on a novel PEG-based resin, Angew. Chem. Int. Ed., 36, 1976-1978, 1997. 

Employing this strategy, a silicon diol warhead moiety was incorporated into the Val-Pro-Val motif however, also realizing that this provided a relatively rigid inhibitor, more flexible analogs were designed around an isoleucine-isobutyl peptide template wherein the isobutyl group would... 

M. K. Christensen, M. Meldal, K. Bock, H. Cordes, S. Mouritsen, H. Eisner. Synthesis of glycosylated peptide templates containing 6 -0- phosphorylaled mannose disaccharides and their binding to the mannose-6-phosphate receptor. J. Chem. Soc., Perkin Trans. 1 1994, 1299-1310. 

M. Meldal, I. Christiansen-Brams. M. K. Christensen, S. Mouritsen, K. Bock Synthesis and biological application of glycosylated peptide templates in Complex carbohydrates in drug research. Structural and functional aspects. (Eds Bock K, Clausen H), Copenhagen, Munksgaard, 1994. pp. 15.3-165. 

In summary, quantitative, selective, and heterogeneous metal assembly was accomplished using a predesigned peptide template. Peptides with the predetermined number and sequence of metal coordination sites would allow template-directed arrays of metal-containing nanosized devices. 

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