Mannitol lactic acid bacteria

Production of Mannitol by Lactic Acid Bacteria A Review... 

Mannitol production by homofermentative lactic acid bacteria 392... 

Mannitol production by heterofermrentative lactic acid bacteria 393... 

PRODUCTION OF MANNITOL BY LACTIC ACID BACTERIA A REVIEW... 

Some microorganisms can specifically produce mannitol from glucose or fructose without making a sorbitol byproduct (Smiley et al., 1967 Song et al., 2002 Wisselink et al, 2002 Saha, 2003). Mannitol, at 180g/L, can be easily recovered from the fermentation broth by cooling crystallization. Thus, research efforts have been directed toward production of mannitol by fermentation and enzymatic means (Vandamme and Soetaert, 1995). In this paper, the authors review the production of mannitol by lactic acid bacteria. 

MANNITOL PRODUCTION BY HOMOFERMENTATIVE LACTIC ACID BACTERIA... 

MANNITOL PRODUCTION BY HETEROFERMENTATIVE LACTIC ACID BACTERIA 393... 

Racine, E, and Saha, B. C. 2006. Fed-batch and continuous production of mannitol by a heterofermentative lactic acid bacteria. In McKeon, M., and Barton, F. II (Eds.), Proc. United States—Japan Cooperative Program in Natural Resources Food and Agriculture Panel Meeting, Sonoma, CA (pp. BT13-BT17). 

G, Hugenholtz, J, et al. 2002. Mannitol production by lactic acid bacteria a review. International Dairy Journal, 12 151-61. 

Carvalheiro, F, Moniz, P, Duarte, L.C., Esteves, M.P. et al. (2011) Mannitol production by lactic acid bacteria grown in supplemented carob syrup, /, Ind. Microbid. Biotechnol, 38, 221-227,... 

Moniz, P., Carvalheiro, F., Moura, P, Pereira, J. et al. (2009) Screening and characterization of lactic acid bacteria for the production of mannitol in carob based syrups. BioMicroWorld2009, Lisbon. 

Wisselink, H. W., Weusthuis, R. A., Eggink, G., Hugenholtz, J., Grobben, G. J. (2002). Mannitol production by lactic acid bacteria a review. International Dairy Journal, 12(2—3), 151-161. 

PiLONE, G.J., M.G. Clayton, and RJ. van Duivenboden. 1991. Characterization of wine lactic acid bacteria Single broth culture for tests of heterofermentation. Mannitol from fructose and ammonia from arginine. Am. J. Enol Vitic. 42(2) 153-157. 

The silages usually contain small amormts of acetic acid and may also contain traces of propionic and butyric acids. Variable amounts of ethanol and mannitol derived from the activities of lactic acid bacteria and yeasts are present. Only very small quantities of water-soluble carbohydrates remain after fermentation, usually less than 20 g/kg DM. 

As stated previously, many heterofermentative lactic acid bacteria gain additional energy by converting acetyl phosphate to acetate instead of ethanol. Although an additional ATP can be produced, the cell requires regeneration of NAD, a process achieved using an alternative electron acceptor, fructose (Wisselink et al., 2002). The reduction of fructose to mannitol by lactic acid bacteria catalyzed by mannitol dehydrogenase is shown in Fig. 2.8. 

Mannitol formation is used as a laboratory diagnostic test for the separation ofheterofermentative from homofermentative bacteria (Section 15.4.10). Although primarily a property ofheterofermentative lactic acid bacteria, a few homofermentative strains can also produce small amounts of the sugar alcohol (Wisselink et al., 2002). 

Mannitol is produced by some lactic acid bacteria (Section 2.4.4) as per... 

Additional yeast spoilage is induced by species of the genera Candida Mycoderma), Pischia and Hansenula (Willia). Other microorganisms are involved in the formation of viscous, moldy and ropy wine flavor defects. Bacterial spoilage may involve acetic acid and lactic acid bacteria. In this case vinegar or lactic acid souring is detectable. It has usually been associated with mannitol fermentation which may result in considerable amounts of mannitol. 

Several heterofermentative LAB belonging to the genera Lactobacillus, Leu-conostoc, and Oenococcus can produce mannitol from fructose effectively (Saha, 2003). In addition to mannitol, these bacteria may produce lactic acid, acetic acid, carbon dioxide, and ethanol. The process is based on the ability of the LAB to use fructose as an electron acceptor and reduce it to mannitol with the participation of the enzyme mannitol 2-dehydrogenase (EC 1.1.1.38). 

Several heterofermentative LAB produce mannitol in large amounts, using fructose as an electron acceptor. Mannitol produced by heterofermentative bacteria is derived from the hexose phosphate pathway (Soetaert et al., 1999 Wisselink et al., 2002). The process makes use of the capability of the bacterium to utilize fructose as an alternative electron acceptor, thereby reducing it to mannitol with the enzyme mannitol dehydrogenase. In this process, the reducing equivalents are generated by conversion of one-third fructose to lactic acid and acetic acid. The enzyme reaction proceeds according to (theoretical) Equation 21.1 ... 

Besides acetic acid, lactic spoilage produces lactic acid and various secondary compounds that contribute to various olfactory defects. Some bacteria convert fructose into mannitol, explaining why this phenomenon used to be known as man-nitic fermentation. 

Lactic acid is mainly prepared in large quantities (around 200 kT per year) by the bacterial fomentation of carbohydrates. These fermentation processes can be classified according to the type of bacteria used (i) the hetero-fermentative method, which produces less than 1.8 mol of lactic acid per mole of hexose, with otho- metabolites in significant quantities, such as acetic acid, ethanol, glycerol, mannitol and carbon dioxide (ii) the homo-fomentative method, which leads to greater yields of lactic acid and lower levels of by-products, and is mainly used in industrial processes [3]. The convo-sion yield from glucose to lactic acid is more than 90 per cent. 

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