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MALDI desorption mass spectrometry

Laser-desorption mass spectrometry (LDMS) or matrix-assisted laser desorption ionization (MALDI) coupled to a time-of-flight analyzer produces protonated or deprotonated molecular ion clusters for peptides and proteins up to masses of several thousand. [Pg.417]

Matrix-assisted laser desorption mass spectrometry (MALDI-MS) is, after electrospray ionization (ESI), the second most commonly used method for ionization of biomolecules in mass spectrometry. Samples are mixed with a UV-absorbing matrix substance and are air-dried on a metal target. Ionization and desorption of intact molecular ions are performed using a UV laser pulse. [Pg.748]

In a separate study, a protocol for Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) has been proposed.18 This IMS technique provides a new approach to visualize spatial distribution of thousands of molecular species, including peptides, proteins, and their metabolites in two- or three-dimensional levels. This approach may also provide a straightforward method of determining the tissue distribution of multiple peptides or proteins in a quantitative manner.18 Chu et al.19 reported a nondestructive molecular extraction method to obtain proteins from a single FFPE or frozen tissue section, without destroying the tissue morphology, such... [Pg.394]

Matrix assisted laser desorption ionization time-of-flight (MALDI-TOE) mass spectrometry was carried out with a PerSeptive Biosystems Voyager-DE-RP MALDl-TOF mass spectrometer. A 337-nm UV nitrogen laser producing 3-ns pulses was used in the reflectron mode. The samples were prepared by mixing 10 pi of a 0.1 M HAc solution of the sample with 20 pi of a solution of 3 mg/1 a-cyano-4-hydroxy cinnamic acid in wafer. One pi of that solution was loaded on the gold-sample plate. [Pg.78]

Schiller, J. Suss, R. Amhold, J. Fuchs, B. Lessig, J. Muller, M. Petkovic, M. Spalteholz, H. Zschomig, O. Arnold, K. Matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry in lipid and phospholipid research. Prog. Lipid Res. 2004, 43, 449-488. [Pg.60]

Finally, another method commonly used to characterize these copolymeric materials is that of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. When using this technique, it is possible to investigate the molecular weight distributions, end groups, and polymer constitution of low-molecular-weight samples [63], MALDI-TOF may also provide vital... [Pg.240]

Interaction of nanomaterials with native cells is an important problem in modem life science. Recent progress in mass spectrometry provides a vital tool to study this problem. Advances in applications of mass spectrometry for investigating the interaction of nanoparticles with cell membranes and biomacromolecules are based on at least two methods. The first is matrix-assisted laser desorption ionization (MALDI),1 and the second is temperature-programmed desorption mass spectrometry (TPD MS), newly developed to study the interaction of nanoparticles with a cell surface.2 ... [Pg.277]

M. K. Fatema, H. Nonami, D. R. B. Ducatti, A. G. Gonqalves, M. E. R. Duarte, M. D. Noseda, A. S. Cerezo, R. Erra-Balsells, and M. C. Matulewicz, Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of oligosaccharides and oligosaccharide alditols obtained by hydrolysis of agaroses and carrageenans, two important types of red seaweed polysaccharides, Carbohydr. Res., 345 (2010) 275-283. [Pg.190]

Concentration detection limits in CE-MS with the ESI interface are similar to those with UV detection. Sample sensitivity can be improved by using ion-trapping or time-of-flight (TOE) mass spectrometers. MS analysis can also be performed off-line, after appropriate sample collection, using plasma desorption-mass spectrometry (PD-MS) or matrix-assisted laser desorption-mass spectrometry (MALDI-MS). [Pg.137]


See other pages where MALDI desorption mass spectrometry is mentioned: [Pg.12]    [Pg.6]    [Pg.435]    [Pg.705]    [Pg.40]    [Pg.239]    [Pg.173]    [Pg.225]    [Pg.63]    [Pg.411]    [Pg.13]    [Pg.21]    [Pg.99]    [Pg.15]    [Pg.362]    [Pg.152]    [Pg.1325]    [Pg.112]    [Pg.69]    [Pg.128]    [Pg.321]    [Pg.19]    [Pg.176]    [Pg.135]    [Pg.411]    [Pg.431]    [Pg.34]    [Pg.407]    [Pg.728]    [Pg.105]    [Pg.586]    [Pg.20]    [Pg.135]    [Pg.500]    [Pg.463]   


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Desorption mass spectrometry

Laser desorption/ionization mass spectrometry MALDI

MALDI

MALDI -mass spectrometry

MALDI desorption ionization mass spectrometry

MALDI spectrometry

Mass spectrometry desorption/ionisation (MALDI

Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry (MALDI-TOF-MS)

Matrix assisted laser desorption ionization MALDI) mass spectrometry

Matrix-Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS)

Matrix-assisted laser desorption ionisation MALDI) mass spectrometry

Matrix-assisted laser desorption mass spectrometry, MALDI

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