Magnusson-Kligman test

Concerning sensitization, sulfosuccinates have been proven nonsensitizing according to the Magnusson-Kligman test [97]. 

Surfactant Acute toxicity Irritation to skin" (rabbit) Irritation to eye (rabbit) Sensitization (Magnusson-Kligman test) Gene mutation (Ames test) NOAEL ... 

Not irritant to skin and mucous membranes. No sensitizing effect was observed in the guinea-pig (Magnusson-Kligman test). 

Table 1.1 Magnusson/Kligman grading scale for the evaluation of challenge patch test reactions [130],...

No sensitization was observed in the guinea pig test according to Magnusson-Kligman — Bacterial mutagenicity tests were negative. 

Magnusson, B. and Kligman, A.M., The identification of contact allergens by animal assay. The guinea pig maximization test, J. Invest. Dermatol., 52, 268, 1969. 

Cell-mediated immunity Cytotoxic T lymphocyte killing delayed type hypersensitivity response mouse ear swelling test (MEST Gad et al., 1986) guinea pig maximization test (Magnusson and Kligman, 1969). 

There are several acceptable ways to evaluate DTH responses in nonclinical species. Of these, the most common are the guinea pig assays used to assess contact sensitization. Both the Magnusson and Kligman model (guinea pig maximization test) and the Buehler model measure the elicitation phase of the hypersensitivity response, though the tests vary in their methods of chemical application and utilization of adjuvants. Most recently, the local lymph node assay has been accepted as a stand alone test for chemical hypersensitivity. This assay is conducted in mice and measures the induction phase of sensitization. In humans, the most common methods to assess delayed hypersensitivity are the patch test (contact sensitivity for diagnostic purposes) and the human repeat insult patch test (contact sensitivity for predictive purposes). Additionally, intradermal... 

The GPMT of Magnusson and Kligman (which uses adjuvant) and the nonadjuvant Buehler test are given preference over other methods and these procedures are described in detail. 

Aluminum was a grade-I sensitizer at animal testing (Magnusson and Kligman 1970). It is commercially available as is and as aluminum chloride hexahy-drate for patch testing (Chemotechnique Diagnostics 1998). 

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