Magnetically stabilized fluidized

Novel Bioparticle Research. Two major thrusts have been seen in recent particle research—the area of density manipulation so that particle density suits the desired fluidization mode, and the development of magnetic particles for use in magnetically stabilized fluidization. Intraparticle mass transfer is also of interest. Table 18 lists several novel particles developed in recent years to address these and other concerns. 

Bramble, J. L., Graves, D. J., and Brodelius, P., Plant Cell Culture Using a Novel Bioreactor The Magnetically Stabilized Fluidized Bed, Biotechnol. Prog.,... 

Terranova, B. E., and Bums, M. A., Continuous Cell Suspension Processing Using Magnetically Stabilized Fluidized BedsBiotechnol. Bioeng., 37 110... 

Bahar, T. and Gelebi, S.S., Performance of immobilized glucoamylase in a magnetically stabilized fluidized bed reactor (MSFBR), Enzyme Microbial Tech., 26 (2000) 28-33. 

Graham LJ, Jovanovic G. Dechlorination of p-chlorophenol on a Pd/Fe catalyst in a magnetically stabilized fluidized bed Implications for sludge and liquid remediation. Chem Eng Sci 1999 54 3085-3093. 

Stable expanded-bed operations promise the ability to handle whole broths efficiently, all the while maintaining plug-flow characteristics. Magnetically stabilized fluidized beds have been shown to work effectively for bioproduct separations, but are not yet used commercially. A commercially available process uses well-designed beads of appropriate densities and sizes to enable bed fluidization and stable operation without appreciable recirculation. 

In an isolation step, where yield and concentration are more important than purity, the adsorption mechanism can be considered an on/off process, and several alternative contacting schemes can be used. Ligands have been bound to magnetized particles (137, 138) for continuous countercurrent adsorption in magnetically stabilized fluidized beds. Ligands attached to liquid perfluorocarbons (143), to dextran and related polymers (144), or incorporated into liposomes (145), or reversed micelles (146) may be used for biospecific liquid-liquid extraction or "affinity partitioning". Ligands have also been attached to surfactants and biopolymers for selective precipitation of dilute protein species (147, 148). 

Lochmuller, C. H. Wigman, L. S. "Affinity Separations in Magnetically Stabilized Fluidized Beds Synthesis and Performance of Packing Materials" Sep. Sci. Tech. 1987, 22, pp 2111-2125. 

A mathematical analysis of a crossflow magnetically stabilized fluidized bed chromatograph has been presented (14). The geometry of this system is similar to the rotating annular chromatograph and therefore the modeling approach is quite similar to that reported here. A parametric sensitivity study was conducted and the results indicated that the extent of band broadening was most sensitive to two factors. These factors were the external resistance to mass transfer and the width of the feed band. 

Recently, continuous production of daizein and genstein from soybean in a magnetically stabilized fluidized bed bioreactor was reported [163]. Brodelius [164] previously demonstrated the use of plant cells in MSFB bioreactor. 

Putnam, D.D. Namasivayam, V. Bums, M.A. Cell affinity separations using magnetical stabilized fluidized beds Er5dbrocyte subpopulation fractionation utilization a lectin-magnetite support. Biotecbnol. Bioeng. 2003, 81, 650. 

Figure 8.3.4. Continuous chromatographic separation of a four-component feed-gas mixture in a magnetically stabilized fluidized bed moving in the z-direction with carrier gas and feed-gas mixture introduced via crossflow in the y-direction. (After Siegell et al. (1986).)...

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