Immobilized glucoamylase

Bahar, T. and Gelebi, S.S., Performance of immobilized glucoamylase in a magnetically stabilized fluidized bed reactor (MSFBR), Enzyme Microbial Tech., 26 (2000) 28-33. 

Shiraishi et al. [49,50] immobilized glucoamylase of Rhizopus delemar in monolith structures and used them for saccharification of soluble starch. The process was studied at first in a batch reactor at SOX and 4.5 bar. The simplified kinetic model was developed. A continuous process was realized in a monolith reactor consisting of 10 pieces stacked on top of each other, where the blocks were rotated by ir/4 on their axes. The reaction rate at a glucose concentration of 460 g dm" was approximately two times higher than in a conventional industrial process. Conversion of 47% was reached at a space time of 12 hr. The half-life of enzyme was 79 days. 

F. Shiraishi, K. Kawakami, and K. Kusunoki, K., Saccharification of starch in an immobilized glucoamylase monolithic reactor, Kagaku Kogaku Ronbunshu 12(4) 492 (1986). 

Active immobilized enzyme heat-transfer study of packed reactors incorporating immobilized glucoamylase Active immobilized enzyme... 

These are glucose-lsomerase, lactase and amlnoacylase. In the GDR specially Immobilized glucoamylase and Invertase are used on an Industrial scale. 

BC can also be employed as support for enzymes and cells immobilization. Glucoamylase has been immobilized in BC beads (0.5-1.5 mm) which demonstrated to increase its stability towards pH and temperature changes [67]. Wine yeast has also been immobilized in BC, showing higher metabolic activity and resistance to imfavor-able conditions during wine fermentation in comparison with non-immobilized yeasts. In addition, the application of immobilized yeast to repeated batch fermentation in wine-making enhances the economic effectiveness of the production-line because of the cost reduction in inoculum preparation and the simple separation of yeast at the end of the fermentation [68, 69]. 

A highly-substituted carboxymethylcellulose gel with a high degree of hydration has been prepared by precipitation from a solution of the sodium salt, treatment with formaldehyde, and freezing. The gel was converted into an azide derivative, which was used to immobilize glucoamylase. A method for determining the d.s. of carboxymethylcelluloses has been improved.227 Carboxymethylcellulose has been used in studies of the action of rennin on K-casein.22 ... 

Reetz MT, Jaeger KE (1998) Overexpression, immobilization and biotechnological application of Pseudomonas lipases. Chem Phys Lipids 93 3-14 Reilly PJ (1979) Starch hydrolysis with soluble and immobilized glucoamylase. 

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