M13 phage

Fig. 9. Mutagenesis by a synthetic oligonucleotide of a cloned sequence available in single-stranded form (a) single-stranded M13 template containing uracil (U) residues (b) double-stranded product, uracil residues are not mutagenic (c) strong selection for M13 phages containing mutation of interest (23).

Yanisch-Perron, C., Vieira, J. and Messing, J. 1985. Improved M13 phage cloning vectors and host strains nucleotide sequences of the M13mpl8 and pUCl9 vectors. Gene 33 103-1 19. 

Fig. 14.8 Measured resonant mode spectral shift for increasing concentration of M13 phage. Reprinted from Ref. 27 with permission. 2008 The Royal Society of Chemistry...

Amplified electrochemical detection of DNA in monolayer assemblies was accomplished by the conjugation of bioelectrocatalytic transformations to the DNA recognition events. This was exemplified with the amplified electrochemical analysis of M13 phage DNA (Fig. 12.20a).75 A capturing nucleic acid, (20), complementary to... 

A major source of human antibodies are phage display libraries, which are constructed from various genetic sources. Antibodies are expressed as scFV and Fab antibody fragments using various vector systems. This review offers a comprehensive overview of M13 phage display antibody vectors and discusses their applications. 

Roberts BL, Markland W, Siranosian K, Saxena, Guterman SK, Ladner RC, Protease inhibitor display M13 phage selection of high-affinity neutrophil, elastase inhibitors, Gene, 121 9-15, 1992. 

Fig. 4.3. Diagram showing the origin of the two single-stranded M13 phage recombinants obtained after cloning a DNA fragment into the double-stranded replicative form of M13.

Figure 6.17. M13 Phage DNA, a Cloning and Sequencing Vector. M13 phage DNA is very useful in sequencing DNA fragments by the dideoxy method. A double-stranded DNA fragment is inserted into Ml 3 RF DNA. Synthesis of new strand is primed by an oligonucleotide that is complementary to a sequence near the inserted DNA.

Ryskov AP, Jincharadze AG, Prosnyak AG, Ivanov PL Limborskaya (1988) M13 phage as a universal marker for DNA fingerprinting of animals, plants and microorganisms. FEES Letters 233 388-392. 

M13 phage DNA, in the form of SS M13mp8 DNA can be labelled by random primer extension using the Multiprime kit (Amersham International) according to the manufacturer s instructions. 

Chen P. Hayward NK, Kidson C Ellem KAO (1990) Conditions for generating well-resolved human DNA fingerprints using M13 phage DNA. Nucleic Acids Research 18 1065. 

Vassart G, Georges M, Monsieur R, Brocas H, Lecarre AS Christophe D (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235 683-684. 

The genetic evidence presented above makes it clear that E. coli, and possibly other bacteria, possess a complex set of proteins that act in the protein-secretion process. Although it appears that at least one protein, the M13 phage coat protein, can be localized and processed in the absence of proteins other than signal peptidase (Section V,B) (Silver et al., 1981 Ohno-Iwashita and Wickner, 1983 Watts et al., 1981), most proteins of the bacterial cell envelope require the participation of a secretion apparatus for proper localization. Whether the bacterial secretion process is analogous to the eukaryotic process remains to be seen. The recent development of in vitro translocation systems derived from E. coli should facilitate research in this area (Rhoads et al., 1984 Muller and Blobel, 1984b). 

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