M13 phage DNA

Amplified electrochemical detection of DNA in monolayer assemblies was accomplished by the conjugation of bioelectrocatalytic transformations to the DNA recognition events. This was exemplified with the amplified electrochemical analysis of M13 phage DNA (Fig. 12.20a).75 A capturing nucleic acid, (20), complementary to... 

Figure 6.17. M13 Phage DNA, a Cloning and Sequencing Vector. M13 phage DNA is very useful in sequencing DNA fragments by the dideoxy method. A double-stranded DNA fragment is inserted into Ml 3 RF DNA. Synthesis of new strand is primed by an oligonucleotide that is complementary to a sequence near the inserted DNA.

M13 phage DNA, in the form of SS M13mp8 DNA can be labelled by random primer extension using the Multiprime kit (Amersham International) according to the manufacturer s instructions. 

Chen P. Hayward NK, Kidson C Ellem KAO (1990) Conditions for generating well-resolved human DNA fingerprints using M13 phage DNA. Nucleic Acids Research 18 1065. 

Ryskov AP, Jincharadze AG, Prosnyak MI, Livanov PL Limborska SA (1988) M13 phage DNA as a universal marker for DNA fingerprinting of animals, plants and micro-organisms. FEES Letters 233 388-392. 

Fig. 4.3. Diagram showing the origin of the two single-stranded M13 phage recombinants obtained after cloning a DNA fragment into the double-stranded replicative form of M13.

Vassart G, Georges M, Monsieur R, Brocas H, Lecarre AS Christophe D (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235 683-684. 

Fig. 5. Generation of mutants using single-stranded DNA. After cloning the target gene into M13, the phage is propagated in the E. coll dut, ung strain of E.

Figure 5.16 Illustration of the manner by which the virion of a filamentous single-stranded phage (such as M13 or fd) leaves an infected cell without lysis. The A protein passes first through the membrane at a site on the membrane where coat protein molecules have first become imbedded. The intracellular circular DNA is coated with dimers of another protein, gp5, which is displaced by coat protein as the DNA passes through the intact membrane.

Another viral chromosome that can be used as a vector is that of the filamentous phage Ml3. The M13 chromosome is a single-stranded DNA molecule which when inserted into the bacterial host replicates outside the bacterial chromosome in the cytoplasm. The virus is then reassembled and released from the bacterial cell without cell lysis. 

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