LNCaP cell line

In contrast to the androgen-independent DU-145 and PC-3 cells, the LNCaP cell line is resistant to the inhibiting effect of TGF (3-1, since it lacks either the T(3RI... 

Mice bearing exponentially growing castration-resistant tumors LNCaP cell line PC3 cell line DU145 cell lines LNCaP cell lines... 

PC3 cell line LNCaP cell line xenograft model... 

LNCaP cell line DU145 cell line PC3 cell 161 line... 

Jarry H, Thelen P, Christoffel V, Spengler B, Wuttke W. Cimicifuga racemosa extract BNO 1055 inhibits proliferation of the human prostate cancer cell line LNCaP. Phytomedicine 2005 12 178-182. 

An elegant study of lycopene uptake in LNCaP, PC-3, and DU-145 cells using beadlet-delivered 1.48 J.M all-trans lycopene (a maximal level in human plasma) found that all three cell lines rapidly took up lycopene during the first 10 h of incubation. Cells continued to accrue lycopene, but more slowly, over the next 48h. The uptake by the LNCaP cells was 2.5-fold higher than PC-3 cells and 4.5-fold higher than DU-145 cells at 24h of incubation but lycopene showed no affinity for the AR receptor, which is expressed in the LNCaP cells (Liu et al. 2006). LNCaP uptake followed Michaelis-Menten kinetics with a V m i, of 66.3pmol/106 cells/h and a Km of 7.72 pM lycopene. Because of the sensitivity of their LC-MS-MS lycopene assay, Liu et al. were also able to investigate the subcellular lycopene distribution. The nuclear membrane contained 55%, the nuclear matrix 26%, and the microsomal fraction 19% of the intracellular fraction. The cytosol contained no lycopene(Liu et al. 2006). 

PTEN deficient cell lines miscible) LycoTrue 48, and Crystal violet Decreased by 60% at 1 pM in LNCaP, PC-3 for LycoTrue... 

Hormonal effects. A commercial product, PC-SPES, composed of Chrysantemum morifolium, Ganoderma lucidum, Glycyrrhiza glabra, Isatis indigotica, Panax pseudoginseng, Rabdosia rubescens, Scutellaria baicalensis, and Serenoa repens was tested in hormone-insensitive cell lines LNCAP-BCL-2, PC-3, and DU-145 at variable concentrations. LNCAP, the only hormone-sensitive cell line, was affected by the lowest dose of PC-SPES tested . 

Chinese hamster ovary cells overexpressing the prolactin receptor, was active . Protein synthesis stimulation. Sterol fraction of the extract, in cell culture at a concentration of 25 (xg/mL, produced weak activity on CA-LNCAP. A concentration of 50 (xg/mL was active on CA-PC3 h PSA production inhibition. Ethanol (70%) extract of PC-SPES (a Chinese herb combination of chrysanthemum, dyers woad, licorice, reishi, san-qi ginseng, rabdosia, saw palmetto, and baikal skullcap), in cultured prostate cancer cell line at variable doses for 24 hours, produced a significant effect in supressing cell growth in all the cell lines h... 

Shi P, Chen WW, Hu XY, Yu CX, Zhang PJ, Jiang AL, Zhang JY. 2006b. Up-regulates the expression of maspin gene in prostate cancer cell line LNCaP. Yao Xue Xue Bao 41 1152-1156. 

CaSR may also influence the proliferative and apoptotic status of the cells indirectly via modulation of cell volume homeostasis. Indeed, stimulation of CaSR in human epithelial cells induces upregulation of volume-regulated anion channels (VRAC) via a G protein-mediated increase in intracellular cAMP (Shimizu, et al., 2000). Proliferation and apoptosis are associated with essential volume perturbations [e.g., (Lang, et al., 2000)] and VRAC, a key component of homeostatic volume regulation, has been directly implicated in proliferation (Chen, et al., 2002, Doroshenko, et al., 2001, Shen, et al., 2000, Wang, et al., 2002) and apoptosis (Lemonnier, et al., 2004, Okada, et al., 2001, Okada, et al., 2006, Shen, et al., 2002). Consequently, extracellular Ca2+ may affect carcinogenesis via the CaSR-VRAC-cell volume links. The Ca2+ -permeable store-operated channel (SOC) is directly and functionally coupled to VRAC in an androgen-dependent LNCaP human prostate cancer epithelial cell line (Lemonnier, et al., 2002), evidence for another, CaSR-unrelated, potential mechanism for extracellular Ca2+ involvement in proliferative and apoptotic events. 

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