Liver L. casei factor

Liver aldehyde oxidase, IX, 119 Liver L. casei factor (N-(4- [(2-amino-4-hydroxy-6-pteridyl)methyl amino) benzoyl]-f(-f-)-glutamic acid), see also under Folic acid and teroylglu-tamic acid... 

Pteroylglutamic acid, see also under Folic acid. Liver L. casei factor, VI, 64,83, 198... 

Synonyms Vitamin Be, norit eluate factor, liver L. casei factor, fola-cin, folic acid. ... 

Liver L. casei factor Liver Pteroylglutamic acid Growth factor for L. casd and S. faecalis antianemic factor for chickens 1943 (552) ... 

The same technique applies to the preparation of vitamin B from horse liver, which is even richer than hog liver in this vitamin (459). The preparation of vitamin B, from yeast is carried out by the same technique, after a treatment of the yeast extract with conjugase. The crystallized product obtained from yeast is identical with that prepared from liver. Stokstad et al. (557) isolated the liver L. casei factor from the precipitate obtained by treating the aqueous liver extract with 85% ethanol. The active substance is separated by successive adsorption on norit A and Superfiltrol, the last eluate being precipitated by baryta and ethanol. The L. casei factor is esterified, and the esterified product is extracted by butanol. After removal of butanol, the substance is dissolved in methanol and chromatographed on a column of Superfiltrol. It is then eluted with 70 % aqueous acetone. The active esterified product thus eluted is pure, and on hydrolysis yields the free acid which crystallizes. The latter is identical to the vitamin Bo of Pfiffner et al. (459). 

L. Casei Factor Stokstad, Hutchings, and Subba Row91 studied the isolation of this factor from liver. Liver extract containing 20,000 units of activity per gram was dissolved in water, adjusted to pH 8.5, heated to 80° C, and calcium chloride added to flocculate the precipitate that formed. The L. casei factor was adsorbed from the filtrate by activated carbon at pH 3.0. The carbon cake was washed with 60% ethanol to remove inert substances and then it was treated with 0.5 N ammonium hydroxide in 60% ethanol at 70° C to elute the active factor. The filtrate, adjusted to pH 1.3, was percolated through a column of Super Filtrol to adsorb the active factor, and the latter was subsequently eluted with 0.5 N ammonium hydroxide in 60% ethanol. 

Vitamin Be Liver Later found to be identical with the L. casei factor On a vitamin B.-free diet results in a macrocytic anemia in chickens 1939 (275) m s... 

L. casei factor Eluate factor , Norite eluate factor ). Snell and Peterson (56) reported in 1940 that, among the substances required by Lactobacillus casei e, was one found in yeast, liver and other natural materials, which could be adsorbed on norite and eluted therefrom with ammoni-acal alcohol. They referred to this substance as the eluate factor , and described methods for its concentration. Stokstad (57) and Hutchings, Bo-honos, and Peterson (58) carried the concentration somewhat further. Hutchings, et al. also described chemical properties of the factor and showed that a concentrate stimulated the growth of several strains of lactic acid... 

Chicks on a diet very similar to the one previously used for the development of factor U deficiency (61) respond by an increase in weight to the administration of L. casei factor from liver (77). 

The order of bacteriostatic potency of the sulfonamides does not agree with their order of effectiveness in producing vitamin E deficiency, biotin deficiency, L. casei factor deficiency, arterial lesions, heart lesions, or hydropic degeneration of liver cells (36, 49, 103). 

PGA in the free state is widely found in nature, particularly in the liver of higher animals, in spinach leaves, and in yeast. The biological properties characteristic of PGA are its ability to stimulate the growth of L. casei (534) and S. faecalis (S. lactis B) (420) and several other bacteria (299) as well as the growth of chickens (298) hyperchromic macrocytic anemia in chickens, due to PGA deficiency, can thus be cured by its administration (276). PGA, unlike the Citrovorum Factor (CF) (see p. 28),... 

Citrovonim factor CF Liver Urine Closely related to 6-formyl-5,6,7,8-tetra-hydropteroyl glutamic acid Growth factor for Leuconostoc dtromrum and, less so, for L. casei and S. faecalis 1948 (507)... 

---