Liquid chromatography mercury

Bettmer, J., Cammann, K., and Robecke, M., Determination of organic ionic lead and mercury species with high-performance liquid chromatography using sulphur reagents, /. Chromatogr., 654, 177, 1993. 

Bloxam et al. [482] used liquid chromatography with an inductively coupled plasma mass spectrometric detector in speciation studies on ppt levels of mercury in seawater. 

Techniques for analysis of different mercury species in biological samples and abiotic materials include atomic absorption, cold vapor atomic fluorescence spectrometry, gas-liquid chromatography with electron capture detection, and inductively coupled plasma mass spectrometry (Lansens etal. 1991 Schintu etal. 1992 Porcella etal. 1995). Methylmercury concentrations in marine biological tissues are detected at concentrations as low as 10 pg Hg/kg tissue using graphite furnace sample preparation techniques and atomic absorption spectrometry (Schintu et al. 1992). 

Lloyd JBF. 1983. High-performance liquid chromatography of organic explosives components with electrochemical detection at a pendant mercury drop electrode. J Chromatogr 257 227-236. 

J. M. Costa-Fernandez, F. Lunzer, R. Pereiro, N. Bordel and A. Sanz-Medel, Direct coupling of high-performance liquid chromatography to microwave-induced plasma atomic emission spectrometry via volatile-species generation and its application to mercury and arsenic speciation, J. Anal. At. Spectrom., 10, 1995, 1019-1025. 

E. Ramalhosa, S. Rio Segade, E. Pereira, C. Vale and A. Duarte, Simple methodology for methylmercury and inorganic mercury determinations by high-performance liquid chromatography-cold vapour atomic fluorescence spectrometry. Anal. Chim. Acta, 448(1-2), 2001, 135-143. 

C. Schickling and J. A. C. Broekaert, Determination of mercury species in gas condensates by online coupled high-performance liquid chromatography and cold-vapor atomic absorption spectrometry, Appl. Organo-met. Chem., 9(1), 1995, 29-36. 

The Aminco Fluoro-Microphotometer (Fig.3.56) is a filter instrument which is easily adaptable to liquid chromatography. The microflow cell has a capacity of 10 jul. A full range of excitation and emission filters are available. This detector has been adapted for use with the Technicon AutoAnalyser. The system uses a mercury lamp as the source and solid-state electronics. 

Thacker [24] reported the design of a miniature flow fluorimeter for liquid chromatography. The body of the fluorimeter was machined from a block of aluminium and contained a low-pressure mercury lamp, an excitation filter, a quartz flow cell, an emission filter, a photomultiplier tube and a photoconducter in order to compensate for fluctuations in lamp intensity. Fluorescence was examined at a direction perpendicular to that of the excitation light. The cell was small enough for it to be attached directly to the end of the column with a minimum dead volume. 

The photocatalytic oxidation of para- and mete-substituted anisoles with UV/ TiOz was studied by Amalric et al. (1996). A 100-mL batch reactor containing aqueous solutions of the anisoles was irradiated with UV light at 340 nm. The light was provided by mercury lamps. Initial concentrations of the anisoles were 0.1 mM/L. The pH of the solution was adjusted to 5.1. Ti02 was added at 2.5 g/L. The samples were irradiated for 80 min. After irradiation, the samples were filtered and concentrations were determined by liquid chromatography. 

C.F. Harrington, The speciation of mercury and organomercury compounds by using high-performance liquid chromatography, Trends Anal. Chem., 12 (2000) 167-178. 

Newsome [6] determined methyl mercury compounds in wheat flour and ground oats by extraction with benzene-formic acid followed by purification and gas-liquid chromatography. Interfering substances were removed from the extracts by column chromatography on silicic acid and partitioning with cysteine acetate solution. The method is sensitive in the 0.01-0.9 ppm range with a recovery of generally better than 95%. 

Figure 4.5 Separation of two trialkyl lead and three organomercury species. Column 1.5mm i.d. x 5cm long flow rate lOOpimirT1 mobile phase 5mM ammonium pentanesulfonate in 20 80 v/v ACN-H20 (pH 3.4) injection volume 2jul, sample size, 40 pg (as Pb) for (Me)3Pb+, 80 pg (as Pb) for (Et)3Pb+ and 2 ng (as Hg) for each of the organomercury species. Taken from Speciation of mercury and lead compounds by microbore column liquid chromatography inductively coupled plasma chromatography mass spectrometry (Shum ef a/. 1992).

Harrington, C.F. and Catterick, T. (1997) Problems encountered during the development of a method for the speciation of mercury and methylmercury by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. /. Anal. At. Spectrom., 12, 1053-1056. 

Shum, S.C.K., Pang, H. and Houk, R.S. (1992) Speciation of mercury and lead compounds by microbore column liquid chromatography inductively coupled plasma chromatography mass spectrometry. Anal. Chem., 64, 2444—2450. 

Bushee, D.S. (1988) Speciation of mercury using liquid chromatography with detection by inductively coupled plasma mass spectrometry. Analyst, 113, 1167-1170. 

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