Liquid chromatography electrospray ionization mass bile acid analysis

Bile acids, alcohols, and side-chain-shortened steroids have been extensively analyzed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) [1-3].The mobile phase usually features water/ methanol or water/acetonitrile containing an organic acid or buffer. As ESI is a concentration-dependent process, maximum sensitivity is obtained with high-concentration, low-volume samples run at low flow rate [26,27]. We have extensively used low-flow-rate LC-ESI-MS to achieve maximum sensitivity for steroid analysis [26], and with the advent of reliable nanoscale liquid chromatography (nano-LC) equipment, this form... 

Ando, M. Kaneko, T. Watanabe, R. Kikuchi, S. Goto, T. lida, T. Hishinuma, T. Mano, N. Goto, J. High sensitive analysis of rat serum bile acid by liquid chromatography/electrospray ionization tandem mass spectrometry. J. Pharm. Biomed. Anal. 2006, 40 (5), 1179-1186. 

Y. Yang, W. J. Griffiths, H. Naza-, and J. SjOvall, Analysis of bile acids and bile alcohols in urine by capillary liquid chromatography-mass spectrometry using fast atom bombardment or electrospray ionization and collision induced dissociation, Biomed. Chromatogr. 11, 240-255 (1997). 

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