Light microscopy reflection imaging

A point light source is imaged onto the specimen by the objective and the transmitted light collected by the collector lens and detected by a broad-area detector in the case of reflection microscopy, the objective lens also serves simultaneously as a collector (see figure Bl.18.10. The resolution is solely detennined by the objective lens, because the collector has no imaging fimction and only collects the transmitted light. The... 

The TSM can be modified for reflected light confocal microscopy (TSRLM). In that case, one Nipkow disk above the objective serves both for the illuminating beams and the reflected image-forming rays. 

Vukjovic et al.199 recently proposed a simple, fast, sensitive, and low-cost procedure based on solid phase spectrophotometric (SPS) and multicomponent analysis by multiple linear regression (MA) to determine traces of heavy metals in pharmaceuticals. Other spectroscopic techniques employed for high-throughput pharmaceutical analysis include laser-induced breakdown spectroscopy (LIBS),200 201 fluorescence spectroscopy,202 204 diffusive reflectance spectroscopy,205 laser-based nephelometry,206 automated polarized light microscopy,207 and laser diffraction and image analysis.208... 

Reflection contrast Reflection-imaging microscopy Field ion microscopy Quantification in gap between light and em microscopies Useful for imaging highly reflective particles such as silver grains in autoradiographs Atomic structure of crystals Immunoelectron Localization of cellular antigens... 

Microscopy Light microscopy involves the use of light rays and lenses to observe magnified images of objects. The magnified image may be formed from transmitted light for transparent materials or from reflected... 

The mechanism of image formation is different from the light microscopy. It is not absorption, reflection, or fluorescence but scattering of the electrons on the atoms forming the specimen. Elastic scattering on the atomic cores provides information about the specimen structure, morphology, and crystallinity. Inelastic scattering on the atomic shells provides information about its chemical composition and even oxidation state. A comprehensive theory and many examples and practical hints can be found in Refs. 30 and 31. 

Reflected light microscopy images of micropatterns on the photomask. All the micropatterns are 10 jim in width. The lighter areas are chrome coated and opaque and do not transmit light. The darker areas are transparent and allows light to pass through, (a) circles, (b) squares, (c) lines and (d) diamonds. 

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